BD PharmingenTM Alexa Fluor 647 Mouse anti-Human CD196 (CCR6)

Description

Flow cytometric analysis of CCR6 expression on stimulated human PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. After stimulation, cells were surface stained with Alexa Fluor 647 anti-human CCR6 Cat. No. 560466/561751 , fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 , and intracellular stained with either FITC anti-human IFN-gamma Cat. No. 554700; left panel , PE anti-human IL-4 Cat. No. 554516; middle panel , or PE anti-human IL-17A Cat. No. 560436; right panel . The dot plots were derived gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of CCR6 expression on stimulated human PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. After stimulation, cells were surface stained with Alexa Fluor 647 anti-human CCR6 Cat. No. 560466/561751 , fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 , and intracellular stained with either FITC anti-human IFN-gamma Cat. No. 554700; left panel , PE anti-human IL-4 Cat. No. 554516; middle panel , or PE anti-human IL-17A Cat. No. 560436; right panel . The dot plots were derived gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of CCR6 expression on stimulated human PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. After stimulation, cells were surface stained with Alexa Fluor 647 anti-human CCR6 Cat. No. 560466/561751 , fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 , and intracellular stained with either FITC anti-human IFN-gamma Cat. No. 554700; left panel , PE anti-human IL-4 Cat. No. 554516; middle panel , or PE anti-human IL-17A Cat. No. 560436; right panel . The dot plots were derived gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACSCalibur System. Show More Flow cytometric analysis of CCR6 expression on stimulated human PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. After stimulation, cells were surface stained with Alexa Fluor 647 anti-human CCR6 Cat. No. 560466/561751 , fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 , and intracellular stained with either FITC anti-human IFN-gamma Cat. No. 554700; left panel , PE anti-human IL-4 Cat. No. 554516; middle panel , or PE anti-human IL-17A Cat. No. 560436; right panel . The dot plots were derived gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACSCalibur System. Show More Flow cytometric analysis of CCR6 expression on stimulated human PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. After stimulation, cells were surface stained with Alexa Fluor 647 anti-human CCR6 Cat. No. 560466/561751 , fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 , and intracellular stained with either FITC anti-human IFN-gamma Cat. No. 554700; left panel , PE anti-human IL-4 Cat. No. 554516; middle panel , or PE anti-human IL-17A Cat. No. 560436; right panel . The dot plots were derived gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of CCR6 expression on stimulated human PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. After stimulation, cells were surface stained with Alexa Fluor 647 anti-human CCR6 Cat. No. 560466/561751 , fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 , and intracellular stained with either FITC anti-human IFN-gamma Cat. No. 554700; left panel , PE anti-human IL-4 Cat. No. 554516; middle panel , or PE anti-human IL-17A Cat. No. 560436; right panel . The dot plots were derived gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACSCalibur System.