BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human CD222

Description

Multiparameter flow cytometric analysis of CD222 expression by human leucocyte populations. Left Panel - Surface Staining: Human whole blood was stained with Alexa Fluor 488 Mouse Anti-Human CD3 antibody Cat. No. 557694 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; Top Plots or Alexa Fluor 647 Mouse Anti-Human CD222 antibody Cat. No. 565105; Bottom Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Two-color flow cytometric dot plots showing the correlated expression of CD222 or Ig Isotype control staining versus side light-scattered signals SSC or CD3 were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes Left Plots or lymphocytes Right Plots , respectively. Right Panel - Surface and Intracellular Staining: Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix cells. The leucocytes were then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 and stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Top Plots or Alexa Fluor 647 Mouse Anti-Human CD222 antibody Bottom Plots . Two-color flow cytometric dot plots showing the correlated expression of CD222 or Ig Isotype control staining versus side light-scattered signals SSC or CD3 were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes Left Plots or lymphocytes Right Plots , respectively. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multiparameter flow cytometric analysis of CD222 expression by human leucocyte populations. Left Panel - Surface Staining: Human whole blood was stained with Alexa Fluor 488 Mouse Anti-Human CD3 antibody Cat. No. 557694 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; Top Plots or Alexa Fluor 647 Mouse Anti-Human CD222 antibody Cat. No. 565105; Bottom Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Two-color flow cytometric dot plots showing the correlated expression of CD222 or Ig Isotype control staining versus side light-scattered signals SSC or CD3 were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes Left Plots or lymphocytes Right Plots , respectively. Right Panel - Surface and Intracellular Staining: Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix cells. The leucocytes were then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 and stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Top Plots or Alexa Fluor 647 Mouse Anti-Human CD222 antibody Bottom Plots . Two-color flow cytometric dot plots showing the correlated expression of CD222 or Ig Isotype control staining versus side light-scattered signals SSC or CD3 were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes Left Plots or lymphocytes Right Plots , respectively. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multiparameter flow cytometric analysis of CD222 expression by human leucocyte populations. Left Panel - Surface Staining: Human whole blood was stained with Alexa Fluor 488 Mouse Anti-Human CD3 antibody Cat. No. 557694 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; Top Plots or Alexa Fluor 647 Mouse Anti-Human CD222 antibody Cat. No. 565105; Bottom Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Two-color flow cytometric dot plots showing the correlated expression of CD222 or Ig Isotype control staining versus side light-scattered signals SSC or CD3 were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes Left Plots or lymphocytes Right Plots , respectively. Right Panel - Surface and Intracellular Staining: Human whole blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix cells. The leucocytes were then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 and stained with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426/562427 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Top Plots or Alexa Fluor 647 Mouse Anti-Human CD222 antibody Bottom Plots . Two-color flow cytometric dot plots showing the correlated expression of CD222 or Ig Isotype control staining versus side light-scattered signals SSC or CD3 were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes Left Plots or lymphocytes Right Plots , respectively. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.