BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human CD45RC

Description

Multiparameter flow cytometric analysis of CD45RC expression on human peripheral blood leucocytes. Human whole blood was stained with PerCP-Cy5.5 Mouse Anti-Human CD4 Cat. No. 560650 and BD Horizon BV421 Mouse Anti-Human CD19 Cat. No. 562440/562441 antibodies, and either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557714; Left Plots or Alexa Fluor 647 Mouse Anti-Human CD45RC antibody Cat. No. 565857; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Left Panel - Two-parameter flow cytometric contour plots showing the correlated expression of CD45RC or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Middle and Right Panels - Two-color flow cytometric contour plots showing the correlated expression of CD4 Middle Panel or CD19 Right Panel versus CD45RC or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Multiparameter flow cytometric analysis of CD45RC expression on human peripheral blood leucocytes. Human whole blood was stained with PerCP-Cy5.5 Mouse Anti-Human CD4 Cat. No. 560650 and BD Horizon BV421 Mouse Anti-Human CD19 Cat. No. 562440/562441 antibodies, and either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557714; Left Plots or Alexa Fluor 647 Mouse Anti-Human CD45RC antibody Cat. No. 565857; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Left Panel - Two-parameter flow cytometric contour plots showing the correlated expression of CD45RC or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Middle and Right Panels - Two-color flow cytometric contour plots showing the correlated expression of CD4 Middle Panel or CD19 Right Panel versus CD45RC or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Show More Multiparameter flow cytometric analysis of CD45RC expression on human peripheral blood leucocytes. Human whole blood was stained with PerCP-Cy5.5 Mouse Anti-Human CD4 Cat. No. 560650 and BD Horizon BV421 Mouse Anti-Human CD19 Cat. No. 562440/562441 antibodies, and either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557714; Left Plots or Alexa Fluor 647 Mouse Anti-Human CD45RC antibody Cat. No. 565857; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Left Panel - Two-parameter flow cytometric contour plots showing the correlated expression of CD45RC or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Middle and Right Panels - Two-color flow cytometric contour plots showing the correlated expression of CD4 Middle Panel or CD19 Right Panel versus CD45RC or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System.