
561983 ·
BDB Bioscience · Cat: 561983
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Description
Flow cytometric analysis of CD63 expression on human peripheral blood platelets. Platelets were isolated from fresh whole blood and activated by Thrombin Sigma-Aldrich, Cat. No.T8885 , and then fixed with 2 % formaldehyde. After wash, the fixed platelets were stained with either Alexa Fluor 647 Mouse Anti-Human CD63 antibody Cat. No. 561983; solid line histogram or with an Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; dashed line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of platelets. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Flow cytometric analysis of CD63 expression on human peripheral blood platelets. Platelets were isolated from fresh whole blood and activated by Thrombin Sigma-Aldrich, Cat. No.T8885 , and then fixed with 2 % formaldehyde. After wash, the fixed platelets were stained with either Alexa Fluor 647 Mouse Anti-Human CD63 antibody Cat. No. 561983; solid line histogram or with an Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; dashed line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of platelets. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Flow cytometric analysis of CD63 expression on human peripheral blood platelets. Platelets were isolated from fresh whole blood and activated by Thrombin Sigma-Aldrich, Cat. No.T8885 , and then fixed with 2 % formaldehyde. After wash, the fixed platelets were stained with either Alexa Fluor 647 Mouse Anti-Human CD63 antibody Cat. No. 561983; solid line histogram or with an Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; dashed line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of platelets. Flow cytometry was performed using a BD LSR II Flow Cytometer System.