BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human CD75/CD75s

Description

Multiparameter flow cytometric analysis of CD75/CD75s expression on human peripheral blood leucocyte populations . Peripheral blood was treated with BD Pharmingen Cell Lysis Buffer Cat. No. 559759 to lyse erythrocytes and washed. The leucocytes were then stained with either Alexa Fluor 647 Mouse IgM, kappa Isotype Control Cat. No. 560806; Left Plot or Alexa Fluor 647 Mouse Anti-Human CD75/CD75s antibody Cat. No. 566350; Right Plot . Flow cytometric contour plots showing the correlated expression of CD75/CD75s or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Two-color flow cytometric analysis of CD75/CD75s expression on human peripheral blood lymphocytes. Peripheral blood was treated with BD Pharmingen Cell Lysis Buffer Cat. No. 559759 to lyse erythrocytes and washed. The leucocytes were then stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441 and either Alexa Fluor 647 Mouse IgM, kappa Isotype Control Cat. No. 560806; Left Plot or Alexa Fluor 647 Mouse Anti-Human CD75/CD75s antibody Cat. No. 566350; Right Plot .The flow cytometric contour plots showing the correlated expression of CD75/CD75s or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Multiparameter flow cytometric analysis of CD75/CD75s expression on human peripheral blood leucocyte populations . Peripheral blood was treated with BD Pharmingen Cell Lysis Buffer Cat. No. 559759 to lyse erythrocytes and washed. The leucocytes were then stained with either Alexa Fluor 647 Mouse IgM, kappa Isotype Control Cat. No. 560806; Left Plot or Alexa Fluor 647 Mouse Anti-Human CD75/CD75s antibody Cat. No. 566350; Right Plot . Flow cytometric contour plots showing the correlated expression of CD75/CD75s or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Show More Two-color flow cytometric analysis of CD75/CD75s expression on human peripheral blood lymphocytes. Peripheral blood was treated with BD Pharmingen Cell Lysis Buffer Cat. No. 559759 to lyse erythrocytes and washed. The leucocytes were then stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441 and either Alexa Fluor 647 Mouse IgM, kappa Isotype Control Cat. No. 560806; Left Plot or Alexa Fluor 647 Mouse Anti-Human CD75/CD75s antibody Cat. No. 566350; Right Plot .The flow cytometric contour plots showing the correlated expression of CD75/CD75s or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Show More Multiparameter flow cytometric analysis of CD75/CD75s expression on human peripheral blood leucocyte populations . Peripheral blood was treated with BD Pharmingen Cell Lysis Buffer Cat. No. 559759 to lyse erythrocytes and washed. The leucocytes were then stained with either Alexa Fluor 647 Mouse IgM, kappa Isotype Control Cat. No. 560806; Left Plot or Alexa Fluor 647 Mouse Anti-Human CD75/CD75s antibody Cat. No. 566350; Right Plot . Flow cytometric contour plots showing the correlated expression of CD75/CD75s or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Two-color flow cytometric analysis of CD75/CD75s expression on human peripheral blood lymphocytes. Peripheral blood was treated with BD Pharmingen Cell Lysis Buffer Cat. No. 559759 to lyse erythrocytes and washed. The leucocytes were then stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441 and either Alexa Fluor 647 Mouse IgM, kappa Isotype Control Cat. No. 560806; Left Plot or Alexa Fluor 647 Mouse Anti-Human CD75/CD75s antibody Cat. No. 566350; Right Plot .The flow cytometric contour plots showing the correlated expression of CD75/CD75s or Ig Isotype control staining versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System.