BD PharmingenTM Alexa Fluor 647 Mouse anti-Human FoxP3

Description

Flow cytometric analysis of FoxP3 on resting PBMC. Human PBMC were stained with FITC Mouse Anti-Human CD4 Cat. No. 555346 and PE Mouse Anti-Human CD25 Cat. No. 555432 simultaneously. Cells were fixed and permeabilized see recommended assay procedure followed by intracellular staining with Alexa 647 anti-human FoxP3 Cat No. 560045 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+ respectively, shown as either FoxP3 vs CD25 left panel or FoxP3 vs CD4 right panel . Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of FoxP3 on resting PBMC. Human PBMC were stained with FITC Mouse Anti-Human CD4 Cat. No. 555346 and PE Mouse Anti-Human CD25 Cat. No. 555432 simultaneously. Cells were fixed and permeabilized see recommended assay procedure followed by intracellular staining with Alexa 647 anti-human FoxP3 Cat No. 560045 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+ respectively, shown as either FoxP3 vs CD25 left panel or FoxP3 vs CD4 right panel . Flow cytometry was performed on a BD FACSCalibur System. Show More Flow cytometric analysis of FoxP3 on resting PBMC. Human PBMC were stained with FITC Mouse Anti-Human CD4 Cat. No. 555346 and PE Mouse Anti-Human CD25 Cat. No. 555432 simultaneously. Cells were fixed and permeabilized see recommended assay procedure followed by intracellular staining with Alexa 647 anti-human FoxP3 Cat No. 560045 . The dot plots were derived from the gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4+ or CD25+ respectively, shown as either FoxP3 vs CD25 left panel or FoxP3 vs CD4 right panel . Flow cytometry was performed on a BD FACSCalibur System.