BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human Granzyme K

Description

Two-color flow cytometric analysis of Granzyme K expression in human peripheral blood leucocytes. Human peripheral blood cells were treated 15 min; room temperature with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The leucocytes were washed and then stained in BD Perm/Wash Buffer Cat. No. 554723 with Alexa Fluor 488 Mouse anti-Human CD8 antibody Cat. No. 557696 and either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365; Left Plot or Alexa Fluor 647 Mouse Anti-Human Granzyme K antibody Cat. No. 566655; Right Plot . Pseudocolor dot plots showing the correlated expression of Granzyme K or Ig Isotype control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Two-color flow cytometric analysis of Granzyme K expression in human peripheral blood leucocytes. Human peripheral blood cells were treated 15 min; room temperature with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The leucocytes were washed and then stained in BD Perm/Wash Buffer Cat. No. 554723 with Alexa Fluor 488 Mouse anti-Human CD8 antibody Cat. No. 557696 and either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365; Left Plot or Alexa Fluor 647 Mouse Anti-Human Granzyme K antibody Cat. No. 566655; Right Plot . Pseudocolor dot plots showing the correlated expression of Granzyme K or Ig Isotype control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Two-color flow cytometric analysis of Granzyme K expression in human peripheral blood leucocytes. Human peripheral blood cells were treated 15 min; room temperature with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The leucocytes were washed and then stained in BD Perm/Wash Buffer Cat. No. 554723 with Alexa Fluor 488 Mouse anti-Human CD8 antibody Cat. No. 557696 and either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365; Left Plot or Alexa Fluor 647 Mouse Anti-Human Granzyme K antibody Cat. No. 566655; Right Plot . Pseudocolor dot plots showing the correlated expression of Granzyme K or Ig Isotype control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Show More Two-color flow cytometric analysis of Granzyme K expression in human peripheral blood leucocytes. Human peripheral blood cells were treated 15 min; room temperature with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The leucocytes were washed and then stained in BD Perm/Wash Buffer Cat. No. 554723 with Alexa Fluor 488 Mouse anti-Human CD8 antibody Cat. No. 557696 and either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365; Left Plot or Alexa Fluor 647 Mouse Anti-Human Granzyme K antibody Cat. No. 566655; Right Plot . Pseudocolor dot plots showing the correlated expression of Granzyme K or Ig Isotype control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Show More Two-color flow cytometric analysis of Granzyme K expression in human peripheral blood leucocytes. Human peripheral blood cells were treated 15 min; room temperature with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The leucocytes were washed and then stained in BD Perm/Wash Buffer Cat. No. 554723 with Alexa Fluor 488 Mouse anti-Human CD8 antibody Cat. No. 557696 and either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365; Left Plot or Alexa Fluor 647 Mouse Anti-Human Granzyme K antibody Cat. No. 566655; Right Plot . Pseudocolor dot plots showing the correlated expression of Granzyme K or Ig Isotype control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Two-color flow cytometric analysis of Granzyme K expression in human peripheral blood leucocytes. Human peripheral blood cells were treated 15 min; room temperature with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The leucocytes were washed and then stained in BD Perm/Wash Buffer Cat. No. 554723 with Alexa Fluor 488 Mouse anti-Human CD8 antibody Cat. No. 557696 and either Alexa Fluor 647 Mouse IgG2a, kappa Isotype Control Cat. No. 565365; Left Plot or Alexa Fluor 647 Mouse Anti-Human Granzyme K antibody Cat. No. 566655; Right Plot . Pseudocolor dot plots showing the correlated expression of Granzyme K or Ig Isotype control staining versus CD8 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System.