BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human Granzyme M

Description

Multiparameter flow cytometric analysis of Granzyme M expression in human peripheral blood leucocytes. Human peripheral blood cells were treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The leucocytes were washed and then stained in BD Perm/Wash Buffer Cat. No. 554723 with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human Granzyme M antibody Cat. No. 566996; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of Granzyme M or Ig Isotype control staining versus side light-scatter SSC signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Two-color flow cytometric analysis of Granzyme M expression in human peripheral blood lymphocytes. Human peripheral blood cells were treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The leucocytes were washed and then stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse anti-Human CD3 Cat. No. 563798/563797 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human Granzyme M antibody Cat. No. 566996; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of CD3 versus Granzyme M or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Multiparameter flow cytometric analysis of Granzyme M expression in human peripheral blood leucocytes. Human peripheral blood cells were treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The leucocytes were washed and then stained in BD Perm/Wash Buffer Cat. No. 554723 with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human Granzyme M antibody Cat. No. 566996; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of Granzyme M or Ig Isotype control staining versus side light-scatter SSC signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Show More Two-color flow cytometric analysis of Granzyme M expression in human peripheral blood lymphocytes. Human peripheral blood cells were treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The leucocytes were washed and then stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse anti-Human CD3 Cat. No. 563798/563797 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human Granzyme M antibody Cat. No. 566996; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of CD3 versus Granzyme M or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Show More Multiparameter flow cytometric analysis of Granzyme M expression in human peripheral blood leucocytes. Human peripheral blood cells were treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The leucocytes were washed and then stained in BD Perm/Wash Buffer Cat. No. 554723 with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human Granzyme M antibody Cat. No. 566996; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of Granzyme M or Ig Isotype control staining versus side light-scatter SSC signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System. Two-color flow cytometric analysis of Granzyme M expression in human peripheral blood lymphocytes. Human peripheral blood cells were treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 to lyse erythrocytes and fix leucocytes. The leucocytes were washed and then stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon BV421 Mouse anti-Human CD3 Cat. No. 563798/563797 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 565571; Left Plot or Alexa Fluor 647 Mouse Anti-Human Granzyme M antibody Cat. No. 566996; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of CD3 versus Granzyme M or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Cell Analyzer System.