BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human IDO1

Description

Multiparameter flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium without Left Column or with Right Column lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm solution Cat. No. 554722 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with Alexa Fluor 488 Mouse Anti-Human CD11b Cat. No. 557701 or 561687 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; Top Row or Alexa Fluor 647 Mouse Anti-Human IDO1 antibody Cat. No. 566648; Bottom Row at 0.06ug/test. Two-color flow cytometric pseudocolor plots showing the correlated expression of IDO1 or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Immunohistofluorescent and Immunohistochemical staining of IDO1 in human spleen. Following antigen retrieval with BD Pharmingen Retrievagen A buffer Cat. No. 550524 , the sections of formalin-fixed, paraffin-embedded tissue were stained as described. The IDO1-positive cells are localized to the white pulp near the central arteries. Original magnification, 20. Top Image: Staining with Alexa Fluor 647 Mouse Anti-Human IDO1 Cat. No. 566648, pseudocolored red and BD Horizon BV421 Mouse Anti-Human CD45 Cat. No. 563879 or 563880, pseudocolored green . Photography was performed on a Molecular Devices standard epifluorescence microscope. Bottom Image: Overnight staining with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 550878, not shown or Purified Mouse Anti-Human IDO1 available by custom order . A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig polyclonal secondary antibody Cat. No. 550337 , Streptavidin HRP Cat. No. 550946 and DAB Cat. No. 550880 and Hematoxylin counterstaining were used. Multiparameter flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium without Left Column or with Right Column lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm solution Cat. No. 554722 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with Alexa Fluor 488 Mouse Anti-Human CD11b Cat. No. 557701 or 561687 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; Top Row or Alexa Fluor 647 Mouse Anti-Human IDO1 antibody Cat. No. 566648; Bottom Row at 0.06ug/test. Two-color flow cytometric pseudocolor plots showing the correlated expression of IDO1 or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Show More Immunohistofluorescent and Immunohistochemical staining of IDO1 in human spleen. Following antigen retrieval with BD Pharmingen Retrievagen A buffer Cat. No. 550524 , the sections of formalin-fixed, paraffin-embedded tissue were stained as described. The IDO1-positive cells are localized to the white pulp near the central arteries. Original magnification, 20. Top Image: Staining with Alexa Fluor 647 Mouse Anti-Human IDO1 Cat. No. 566648, pseudocolored red and BD Horizon BV421 Mouse Anti-Human CD45 Cat. No. 563879 or 563880, pseudocolored green . Photography was performed on a Molecular Devices standard epifluorescence microscope. Bottom Image: Overnight staining with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 550878, not shown or Purified Mouse Anti-Human IDO1 available by custom order . A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig polyclonal secondary antibody Cat. No. 550337 , Streptavidin HRP Cat. No. 550946 and DAB Cat. No. 550880 and Hematoxylin counterstaining were used. Show More Multiparameter flow cytometric analysis of IDO1 expression on human peripheral blood mononuclear cells PBMCs . PBMCs were cultured in complete tissue culture medium without Left Column or with Right Column lipopolysaccharide LPS 1 ug/ml, 20-24h, 37C . The cells were harvested, then fixed and permeabilized using BD Cytofix/Cytoperm solution Cat. No. 554722 and BD Perm/Wash buffer Cat. No. 554723 . The cells were then stained with Alexa Fluor 488 Mouse Anti-Human CD11b Cat. No. 557701 or 561687 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; Top Row or Alexa Fluor 647 Mouse Anti-Human IDO1 antibody Cat. No. 566648; Bottom Row at 0.06ug/test. Two-color flow cytometric pseudocolor plots showing the correlated expression of IDO1 or Ig Isotype control staining versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable PBMCs. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Immunohistofluorescent and Immunohistochemical staining of IDO1 in human spleen. Following antigen retrieval with BD Pharmingen Retrievagen A buffer Cat. No. 550524 , the sections of formalin-fixed, paraffin-embedded tissue were stained as described. The IDO1-positive cells are localized to the white pulp near the central arteries. Original magnification, 20. Top Image: Staining with Alexa Fluor 647 Mouse Anti-Human IDO1 Cat. No. 566648, pseudocolored red and BD Horizon BV421 Mouse Anti-Human CD45 Cat. No. 563879 or 563880, pseudocolored green . Photography was performed on a Molecular Devices standard epifluorescence microscope. Bottom Image: Overnight staining with either Purified Mouse IgG1 kappa Isotype Control Cat. No. 550878, not shown or Purified Mouse Anti-Human IDO1 available by custom order . A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig polyclonal secondary antibody Cat. No. 550337 , Streptavidin HRP Cat. No. 550946 and DAB Cat. No. 550880 and Hematoxylin counterstaining were used.