BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human Ikaros

Description

Two-color flow cytometric analysis of Ikaros expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808/561809 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; Left Panel or Alexa Fluor 647 Mouse Anti-Human Ikaros antibody Cat. No. 564868; Right Panel . Two-color flow cytometric contour plots showing the correlated expression patterns of Ikaros or Ig Isotype control staining versus CD3 were generated for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Ikaros expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808/561809 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; Left Panel or Alexa Fluor 647 Mouse Anti-Human Ikaros antibody Cat. No. 564868; Right Panel . Two-color flow cytometric contour plots showing the correlated expression patterns of Ikaros or Ig Isotype control staining versus CD3 were generated for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Ikaros expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808/561809 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; Left Panel or Alexa Fluor 647 Mouse Anti-Human Ikaros antibody Cat. No. 564868; Right Panel . Two-color flow cytometric contour plots showing the correlated expression patterns of Ikaros or Ig Isotype control staining versus CD3 were generated for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of Ikaros expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808/561809 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; Left Panel or Alexa Fluor 647 Mouse Anti-Human Ikaros antibody Cat. No. 564868; Right Panel . Two-color flow cytometric contour plots showing the correlated expression patterns of Ikaros or Ig Isotype control staining versus CD3 were generated for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of Ikaros expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808/561809 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; Left Panel or Alexa Fluor 647 Mouse Anti-Human Ikaros antibody Cat. No. 564868; Right Panel . Two-color flow cytometric contour plots showing the correlated expression patterns of Ikaros or Ig Isotype control staining versus CD3 were generated for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Ikaros expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with PE Mouse Anti-Human CD3 antibody Cat. No. 555333/561808/561809 . The cells were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; Left Panel or Alexa Fluor 647 Mouse Anti-Human Ikaros antibody Cat. No. 564868; Right Panel . Two-color flow cytometric contour plots showing the correlated expression patterns of Ikaros or Ig Isotype control staining versus CD3 were generated for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.