BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human IL-10Rbeta (CD210b)

Description

Multiparameter flow cytometric analysis of IL-10Rbeta CD210b expression on human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human HLA-DR Cat. No. 555812/556644/560943 and FITC Mouse Anti-Human CD14 Cat. No. 555397/557153/561712 antibodies and either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557714 or Alexa Fluor 647 Mouse Anti-Human IL-10Rbeta CD210b antibody Cat. No. 564372 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Panel 1. Two-parameter contour plots show the correlated expression of isotype control Left Plot or IL-10Rbeta CD210b Right Plot versus side-light scatter SSC-A characteristics for gated events with the forward light scatter properties of total viable leucocytes. Panel 2. For gated events with the forward and side-light scatter characteristics of viable mononuclear leucocytes, the two-color contour plots show the correlated expression of CD14 Left Plot or IL-10Rbeta CD210b Right Plot versus HLA-DR for HLA-DR+ and HLA-DR- lymphocytes and CD14+HLA-DR+ monocytes. Multiparameter flow cytometric analysis of IL-10Rbeta CD210b expression on human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human HLA-DR Cat. No. 555812/556644/560943 and FITC Mouse Anti-Human CD14 Cat. No. 555397/557153/561712 antibodies and either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557714 or Alexa Fluor 647 Mouse Anti-Human IL-10Rbeta CD210b antibody Cat. No. 564372 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Panel 1. Two-parameter contour plots show the correlated expression of isotype control Left Plot or IL-10Rbeta CD210b Right Plot versus side-light scatter SSC-A characteristics for gated events with the forward light scatter properties of total viable leucocytes. Panel 2. For gated events with the forward and side-light scatter characteristics of viable mononuclear leucocytes, the two-color contour plots show the correlated expression of CD14 Left Plot or IL-10Rbeta CD210b Right Plot versus HLA-DR for HLA-DR+ and HLA-DR- lymphocytes and CD14+HLA-DR+ monocytes. Show More Multiparameter flow cytometric analysis of IL-10Rbeta CD210b expression on human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human HLA-DR Cat. No. 555812/556644/560943 and FITC Mouse Anti-Human CD14 Cat. No. 555397/557153/561712 antibodies and either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557714 or Alexa Fluor 647 Mouse Anti-Human IL-10Rbeta CD210b antibody Cat. No. 564372 . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Panel 1. Two-parameter contour plots show the correlated expression of isotype control Left Plot or IL-10Rbeta CD210b Right Plot versus side-light scatter SSC-A characteristics for gated events with the forward light scatter properties of total viable leucocytes. Panel 2. For gated events with the forward and side-light scatter characteristics of viable mononuclear leucocytes, the two-color contour plots show the correlated expression of CD14 Left Plot or IL-10Rbeta CD210b Right Plot versus HLA-DR for HLA-DR+ and HLA-DR- lymphocytes and CD14+HLA-DR+ monocytes.