BD PharmingenTM Alexa Fluor 647 Mouse anti-Human IL-17A

Description

Flow cytometric analysis of Alexa Fluor 647 anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with Alexa Fluor 647 anti-human IL-17A, PE-Cy5 anti-human CD4 Cat. No. 555348 and FITC anti-human IFN-gamma Cat. No. 554700; Left Panel or PE anti-human IL-4 Cat. No. 554516; Right Panel . The dot plots were derived from a CD4+ lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of Alexa Fluor 647 anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with Alexa Fluor 647 anti-human IL-17A, PE-Cy5 anti-human CD4 Cat. No. 555348 and FITC anti-human IFN-gamma Cat. No. 554700; Left Panel or PE anti-human IL-4 Cat. No. 554516; Right Panel . The dot plots were derived from a CD4+ lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of Alexa Fluor 647 anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with Alexa Fluor 647 anti-human IL-17A, PE-Cy5 anti-human CD4 Cat. No. 555348 and FITC anti-human IFN-gamma Cat. No. 554700; Left Panel or PE anti-human IL-4 Cat. No. 554516; Right Panel . The dot plots were derived from a CD4+ lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System. Show More Flow cytometric analysis of Alexa Fluor 647 anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with Alexa Fluor 647 anti-human IL-17A, PE-Cy5 anti-human CD4 Cat. No. 555348 and FITC anti-human IFN-gamma Cat. No. 554700; Left Panel or PE anti-human IL-4 Cat. No. 554516; Right Panel . The dot plots were derived from a CD4+ lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System. Show More Flow cytometric analysis of Alexa Fluor 647 anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with Alexa Fluor 647 anti-human IL-17A, PE-Cy5 anti-human CD4 Cat. No. 555348 and FITC anti-human IFN-gamma Cat. No. 554700; Left Panel or PE anti-human IL-4 Cat. No. 554516; Right Panel . The dot plots were derived from a CD4+ lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System. Flow cytometric analysis of Alexa Fluor 647 anti-human IL-17A on stimulated PBMC. Human PBMC were stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours. Cells were then fixed and permeabilized using BD Cytofix/Cytoperm reagents Cat. No. 554714 followed by staining with Alexa Fluor 647 anti-human IL-17A, PE-Cy5 anti-human CD4 Cat. No. 555348 and FITC anti-human IFN-gamma Cat. No. 554700; Left Panel or PE anti-human IL-4 Cat. No. 554516; Right Panel . The dot plots were derived from a CD4+ lymphocyte gate. Flow cytometry was performed on a BD FACSCalibur System.