BD PharmingenTM Alexa Fluor 647 Mouse anti-Human IL-21

Description

Flow cytometric analysis of Alexa 647 anti-human IL-21 on stimulated and non-stimulated PBMC. Human PBMC were cultured with media alone left panel or stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours right panel . Cells were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and stored in BD Pharmingen Stain Buffer FBS Cat. No. 554656 at 4C overnight. The cells were then permeabilized using BD Perm/Wash Buffer Cat. No. 554723 followed by staining with FITC Mouse Anti-Human CD4 clone RPA-T4, Cat. No. 555346 and Alexa 647 Mouse anti-Human IL-21. The dot plots were derived from gated lymphocytes. Flow cytometry was performed on a BD FACSCalibur Flow Cytometer. Flow cytometric analysis of Alexa 647 anti-human IL-21 on stimulated and non-stimulated PBMC. Human PBMC were cultured with media alone left panel or stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours right panel . Cells were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and stored in BD Pharmingen Stain Buffer FBS Cat. No. 554656 at 4C overnight. The cells were then permeabilized using BD Perm/Wash Buffer Cat. No. 554723 followed by staining with FITC Mouse Anti-Human CD4 clone RPA-T4, Cat. No. 555346 and Alexa 647 Mouse anti-Human IL-21. The dot plots were derived from gated lymphocytes. Flow cytometry was performed on a BD FACSCalibur Flow Cytometer. Flow cytometric analysis of Alexa 647 anti-human IL-21 on stimulated and non-stimulated PBMC. Human PBMC were cultured with media alone left panel or stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours right panel . Cells were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and stored in BD Pharmingen Stain Buffer FBS Cat. No. 554656 at 4C overnight. The cells were then permeabilized using BD Perm/Wash Buffer Cat. No. 554723 followed by staining with FITC Mouse Anti-Human CD4 clone RPA-T4, Cat. No. 555346 and Alexa 647 Mouse anti-Human IL-21. The dot plots were derived from gated lymphocytes. Flow cytometry was performed on a BD FACSCalibur Flow Cytometer. Show More Flow cytometric analysis of Alexa 647 anti-human IL-21 on stimulated and non-stimulated PBMC. Human PBMC were cultured with media alone left panel or stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours right panel . Cells were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and stored in BD Pharmingen Stain Buffer FBS Cat. No. 554656 at 4C overnight. The cells were then permeabilized using BD Perm/Wash Buffer Cat. No. 554723 followed by staining with FITC Mouse Anti-Human CD4 clone RPA-T4, Cat. No. 555346 and Alexa 647 Mouse anti-Human IL-21. The dot plots were derived from gated lymphocytes. Flow cytometry was performed on a BD FACSCalibur Flow Cytometer. Show More Flow cytometric analysis of Alexa 647 anti-human IL-21 on stimulated and non-stimulated PBMC. Human PBMC were cultured with media alone left panel or stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours right panel . Cells were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and stored in BD Pharmingen Stain Buffer FBS Cat. No. 554656 at 4C overnight. The cells were then permeabilized using BD Perm/Wash Buffer Cat. No. 554723 followed by staining with FITC Mouse Anti-Human CD4 clone RPA-T4, Cat. No. 555346 and Alexa 647 Mouse anti-Human IL-21. The dot plots were derived from gated lymphocytes. Flow cytometry was performed on a BD FACSCalibur Flow Cytometer. Flow cytometric analysis of Alexa 647 anti-human IL-21 on stimulated and non-stimulated PBMC. Human PBMC were cultured with media alone left panel or stimulated with PMA/Ionomycin in the presence of BD GolgiStop Cat. No. 554724 for 5 hours right panel . Cells were then fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , and stored in BD Pharmingen Stain Buffer FBS Cat. No. 554656 at 4C overnight. The cells were then permeabilized using BD Perm/Wash Buffer Cat. No. 554723 followed by staining with FITC Mouse Anti-Human CD4 clone RPA-T4, Cat. No. 555346 and Alexa 647 Mouse anti-Human IL-21. The dot plots were derived from gated lymphocytes. Flow cytometry was performed on a BD FACSCalibur Flow Cytometer.