BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human MCP-1

Description

Two-color flow cytometric analysis of MCP-1 expression in human periphereal blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma Cat. No. 554617/554616; 10 ng/ml and lipopolysaccharide Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with FITC Mouse Anti-Human CD14 antibody Cat. No. 555397/557153/561712 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557732; Left Panel or Alexa Fluor 647 Mouse Anti-Human MCP-1 antibody Cat. No. 563496; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of MCP-1 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of MCP-1 expression in human periphereal blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma Cat. No. 554617/554616; 10 ng/ml and lipopolysaccharide Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with FITC Mouse Anti-Human CD14 antibody Cat. No. 555397/557153/561712 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557732; Left Panel or Alexa Fluor 647 Mouse Anti-Human MCP-1 antibody Cat. No. 563496; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of MCP-1 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of MCP-1 expression in human periphereal blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma Cat. No. 554617/554616; 10 ng/ml and lipopolysaccharide Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with FITC Mouse Anti-Human CD14 antibody Cat. No. 555397/557153/561712 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557732; Left Panel or Alexa Fluor 647 Mouse Anti-Human MCP-1 antibody Cat. No. 563496; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of MCP-1 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of MCP-1 expression in human periphereal blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma Cat. No. 554617/554616; 10 ng/ml and lipopolysaccharide Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with FITC Mouse Anti-Human CD14 antibody Cat. No. 555397/557153/561712 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557732; Left Panel or Alexa Fluor 647 Mouse Anti-Human MCP-1 antibody Cat. No. 563496; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of MCP-1 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of MCP-1 expression in human periphereal blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma Cat. No. 554617/554616; 10 ng/ml and lipopolysaccharide Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with FITC Mouse Anti-Human CD14 antibody Cat. No. 555397/557153/561712 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557732; Left Panel or Alexa Fluor 647 Mouse Anti-Human MCP-1 antibody Cat. No. 563496; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of MCP-1 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of MCP-1 expression in human periphereal blood mononuclear cells. Human PBMC were cultured 6 hr with Recombinant Human IFN-gamma Cat. No. 554617/554616; 10 ng/ml and lipopolysaccharide Sigma, Cat. No. L-8274; 1.0 mug/ml in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with FITC Mouse Anti-Human CD14 antibody Cat. No. 555397/557153/561712 . After washing, the cells were fixed and permeabilized with BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were then stained in BD Perm/Wash Buffer Cat. No. 554723 with either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557732; Left Panel or Alexa Fluor 647 Mouse Anti-Human MCP-1 antibody Cat. No. 563496; Right Panel using BD Biosciences Intracellular Cytokine Staining protocol. Two-color flow cytometric dot plots show the correlated expression patterns of MCP-1 or Ig Isotype control staining versus CD14 for gated events with the forward and side light-scatter characteristics of intact stimulated PBMC. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.