BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human MNDA

Description

Analyses of MNDA Expression Panel 1. Human Peripheral Blood Leucocytes. Blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to remove erythrocytes and then treated with BD Pharmingen Transcription Factor Phospho Buffer Set Cat. No. 563239 to fix and permeabilize cells. Cells were stained with Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; Left Plot or Alexa Fluor 647 Mouse Anti-Human MNDA Cat. No. 566582; Right Plot at 0.06 mug/test. Panel 2. Human U937 Cells. Human U937 Histiocytic lymphoma, ATCC CRL-1593 cells were fixed and permeabilized with BD Pharmingen Transcription Factor Buffer Set Cat. No. 562725 and stained with Alexa Fluor 647 Mouse IgG1, kappa Isotype Control dashed line histogram or Alexa Fluor 647 Mouse Anti-Human MNDA solid line histogram at 0.5 mug/test. Flow cytometric figures showing the expression of MNDA or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analyses were performed using a BD LSRFortessa X-20 Flow Cytometer System. Panel 3. Human Spleen. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , a section from formalin-fixed, paraffin-embedded human spleen was stained with Alexa Fluor 647 Mouse Anti-MNDA Cat. No.566582 and BD Horizon BV421 Mouse Anti-Human CD45 Cat. No. 563879 antibodies at 1.0 ug/test. Immunohistofluorescent staining of MNDA revealed nuclear staining pseudocolored red of some cells that also showed expression of plasma membrane CD45 pseudocolored green . The image was generated using a standard epifluorescence microscope. Original magnification, 20x. Data shown on this Technical Data Sheet are not lot specific. Analyses of MNDA Expression Panel 1. Human Peripheral Blood Leucocytes. Blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to remove erythrocytes and then treated with BD Pharmingen Transcription Factor Phospho Buffer Set Cat. No. 563239 to fix and permeabilize cells. Cells were stained with Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; Left Plot or Alexa Fluor 647 Mouse Anti-Human MNDA Cat. No. 566582; Right Plot at 0.06 mug/test. Panel 2. Human U937 Cells. Human U937 Histiocytic lymphoma, ATCC CRL-1593 cells were fixed and permeabilized with BD Pharmingen Transcription Factor Buffer Set Cat. No. 562725 and stained with Alexa Fluor 647 Mouse IgG1, kappa Isotype Control dashed line histogram or Alexa Fluor 647 Mouse Anti-Human MNDA solid line histogram at 0.5 mug/test. Flow cytometric figures showing the expression of MNDA or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analyses were performed using a BD LSRFortessa X-20 Flow Cytometer System. Panel 3. Human Spleen. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , a section from formalin-fixed, paraffin-embedded human spleen was stained with Alexa Fluor 647 Mouse Anti-MNDA Cat. No.566582 and BD Horizon BV421 Mouse Anti-Human CD45 Cat. No. 563879 antibodies at 1.0 ug/test. Immunohistofluorescent staining of MNDA revealed nuclear staining pseudocolored red of some cells that also showed expression of plasma membrane CD45 pseudocolored green . The image was generated using a standard epifluorescence microscope. Original magnification, 20x. Data shown on this Technical Data Sheet are not lot specific. Show More Analyses of MNDA Expression Panel 1. Human Peripheral Blood Leucocytes. Blood was treated with BD Pharm Lyse Lysing Buffer Cat. No. 555899 to remove erythrocytes and then treated with BD Pharmingen Transcription Factor Phospho Buffer Set Cat. No. 563239 to fix and permeabilize cells. Cells were stained with Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; Left Plot or Alexa Fluor 647 Mouse Anti-Human MNDA Cat. No. 566582; Right Plot at 0.06 mug/test. Panel 2. Human U937 Cells. Human U937 Histiocytic lymphoma, ATCC CRL-1593 cells were fixed and permeabilized with BD Pharmingen Transcription Factor Buffer Set Cat. No. 562725 and stained with Alexa Fluor 647 Mouse IgG1, kappa Isotype Control dashed line histogram or Alexa Fluor 647 Mouse Anti-Human MNDA solid line histogram at 0.5 mug/test. Flow cytometric figures showing the expression of MNDA or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analyses were performed using a BD LSRFortessa X-20 Flow Cytometer System. Panel 3. Human Spleen. Following antigen retrieval with BD Retrievagen A Buffer Cat. No. 550524 , a section from formalin-fixed, paraffin-embedded human spleen was stained with Alexa Fluor 647 Mouse Anti-MNDA Cat. No.566582 and BD Horizon BV421 Mouse Anti-Human CD45 Cat. No. 563879 antibodies at 1.0 ug/test. Immunohistofluorescent staining of MNDA revealed nuclear staining pseudocolored red of some cells that also showed expression of plasma membrane CD45 pseudocolored green . The image was generated using a standard epifluorescence microscope. Original magnification, 20x. Data shown on this Technical Data Sheet are not lot specific.