BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human Siglec-1 (CD169)

Description

Flow cytometric analysis of Siglec-1 CD169 expression on fresh or Interferon-alpha IFN-alpha -treated human peripheral blood mononuclear cells. Fresh human PBMC Top Panels or PBMC cultured with recombinant human IFN-alpha2a protein 20 ng/mL for 48 hoursor Bottom Panels were prepared. The cells were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 , and stained with PE Mouse Anti-Human CD14 antibody Cat. No. 555398/557154/561707 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; Left Panels or Alexa Fluor 647 Mouse Anti-Human Siglec-1 CD169 antibody Cat. No. 565295; Right Panels . Two-color flow cytometric contour plots showing the correlated expression of Siglec-1 CD169 or Ig Isotype control staining versus CD14 were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of Siglec-1 CD169 expression on fresh or Interferon-alpha IFN-alpha -treated human peripheral blood mononuclear cells. Fresh human PBMC Top Panels or PBMC cultured with recombinant human IFN-alpha2a protein 20 ng/mL for 48 hoursor Bottom Panels were prepared. The cells were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 , and stained with PE Mouse Anti-Human CD14 antibody Cat. No. 555398/557154/561707 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; Left Panels or Alexa Fluor 647 Mouse Anti-Human Siglec-1 CD169 antibody Cat. No. 565295; Right Panels . Two-color flow cytometric contour plots showing the correlated expression of Siglec-1 CD169 or Ig Isotype control staining versus CD14 were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of Siglec-1 CD169 expression on fresh or Interferon-alpha IFN-alpha -treated human peripheral blood mononuclear cells. Fresh human PBMC Top Panels or PBMC cultured with recombinant human IFN-alpha2a protein 20 ng/mL for 48 hoursor Bottom Panels were prepared. The cells were washed, preincubated with BD Pharmingen Human BD Fc Block Cat. No. 564219/564220 , and stained with PE Mouse Anti-Human CD14 antibody Cat. No. 555398/557154/561707 and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; Left Panels or Alexa Fluor 647 Mouse Anti-Human Siglec-1 CD169 antibody Cat. No. 565295; Right Panels . Two-color flow cytometric contour plots showing the correlated expression of Siglec-1 CD169 or Ig Isotype control staining versus CD14 were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.