BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human Sox1

Description

Analysis of Sox1 in H9 Embryonic Stem ES cells left panel and H9-derived Neural Stem Cells NSC right panel . H9 human ES cells WiCell, Madison, WI and H9-derived NSC were harvested with BD Accutase Cell Detachment Solution Cat. No. 561527 , fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No.558050 . Cells were stained with matching concentrations of either Alexa Fluor 647 Mouse Anti-Human Sox1 antibody solid lines or Alexa Fluor 647 Mouse IgG1, kappa isotype control dashed lines, Cat. No.557783 . Histograms were derived from gated events based on light scattering characteristics for the ES and NSC respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. Analysis of Sox1 in H9 Embryonic Stem ES cells left panel and H9-derived Neural Stem Cells NSC right panel . H9 human ES cells WiCell, Madison, WI and H9-derived NSC were harvested with BD Accutase Cell Detachment Solution Cat. No. 561527 , fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No.558050 . Cells were stained with matching concentrations of either Alexa Fluor 647 Mouse Anti-Human Sox1 antibody solid lines or Alexa Fluor 647 Mouse IgG1, kappa isotype control dashed lines, Cat. No.557783 . Histograms were derived from gated events based on light scattering characteristics for the ES and NSC respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. Analysis of Sox1 in H9 Embryonic Stem ES cells left panel and H9-derived Neural Stem Cells NSC right panel . H9 human ES cells WiCell, Madison, WI and H9-derived NSC were harvested with BD Accutase Cell Detachment Solution Cat. No. 561527 , fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No.558050 . Cells were stained with matching concentrations of either Alexa Fluor 647 Mouse Anti-Human Sox1 antibody solid lines or Alexa Fluor 647 Mouse IgG1, kappa isotype control dashed lines, Cat. No.557783 . Histograms were derived from gated events based on light scattering characteristics for the ES and NSC respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Analysis of Sox1 in H9 Embryonic Stem ES cells left panel and H9-derived Neural Stem Cells NSC right panel . H9 human ES cells WiCell, Madison, WI and H9-derived NSC were harvested with BD Accutase Cell Detachment Solution Cat. No. 561527 , fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No.558050 . Cells were stained with matching concentrations of either Alexa Fluor 647 Mouse Anti-Human Sox1 antibody solid lines or Alexa Fluor 647 Mouse IgG1, kappa isotype control dashed lines, Cat. No.557783 . Histograms were derived from gated events based on light scattering characteristics for the ES and NSC respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Analysis of Sox1 in H9 Embryonic Stem ES cells left panel and H9-derived Neural Stem Cells NSC right panel . H9 human ES cells WiCell, Madison, WI and H9-derived NSC were harvested with BD Accutase Cell Detachment Solution Cat. No. 561527 , fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No.558050 . Cells were stained with matching concentrations of either Alexa Fluor 647 Mouse Anti-Human Sox1 antibody solid lines or Alexa Fluor 647 Mouse IgG1, kappa isotype control dashed lines, Cat. No.557783 . Histograms were derived from gated events based on light scattering characteristics for the ES and NSC respectively. Flow cytometry was performed on a BD LSR II flow cytometry system. Analysis of Sox1 in H9 Embryonic Stem ES cells left panel and H9-derived Neural Stem Cells NSC right panel . H9 human ES cells WiCell, Madison, WI and H9-derived NSC were harvested with BD Accutase Cell Detachment Solution Cat. No. 561527 , fixed in BD Cytofix Fixation Buffer Cat. No. 554655 , and permeabilized with BD Phosflow Perm Buffer III Cat. No.558050 . Cells were stained with matching concentrations of either Alexa Fluor 647 Mouse Anti-Human Sox1 antibody solid lines or Alexa Fluor 647 Mouse IgG1, kappa isotype control dashed lines, Cat. No.557783 . Histograms were derived from gated events based on light scattering characteristics for the ES and NSC respectively. Flow cytometry was performed on a BD LSR II flow cytometry system.