BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human STING

Description

Multiparameter flow cytometric analysis of human STING expression in human peripheral blood leukocytes. Human blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 followed by permeablization with BD Perm/Wash Buffer Cat. No. 554723 . Cells were then stained with BD Horizon BV421 Mouse Anti-Human CD20 antibody Cat. No. 563346 and either Alexa Fluor 647 IgG1, kappa Isotype Control Cat. No. 565571, Top Panels or Alexa Fluor647 Mouse Anti-Human STING antibody Cat. No. 564836; Bottom Panels . Left Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus Side Light Scatter SSC were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus CD20 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Multiparameter flow cytometric analysis of human STING expression in human peripheral blood leukocytes. Human blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 followed by permeablization with BD Perm/Wash Buffer Cat. No. 554723 . Cells were then stained with BD Horizon BV421 Mouse Anti-Human CD20 antibody Cat. No. 563346 and either Alexa Fluor 647 IgG1, kappa Isotype Control Cat. No. 565571, Top Panels or Alexa Fluor647 Mouse Anti-Human STING antibody Cat. No. 564836; Bottom Panels . Left Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus Side Light Scatter SSC were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus CD20 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Show More Multiparameter flow cytometric analysis of human STING expression in human peripheral blood leukocytes. Human blood was treated with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 followed by permeablization with BD Perm/Wash Buffer Cat. No. 554723 . Cells were then stained with BD Horizon BV421 Mouse Anti-Human CD20 antibody Cat. No. 563346 and either Alexa Fluor 647 IgG1, kappa Isotype Control Cat. No. 565571, Top Panels or Alexa Fluor647 Mouse Anti-Human STING antibody Cat. No. 564836; Bottom Panels . Left Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus Side Light Scatter SSC were derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Right Panels - Contour plots showing the correlated expression of STING or Ig Isotype control staining versus CD20 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System.