BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human TCL1

Description

Analyses of TCL1 expression Left Panel - Two-color flow cytometric analysis of TCL1 expression in human peripheral blood lymphocytes PBL . Human peripheral blood mononuclear cells were stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441 . The cells were washed, fixed, and permeabilized with the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . Cells were then stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; Left Plot or Alexa Fluor 647 Mouse Anti-Human TCL1 antibody Cat. No. 565856; Right Plot . Two-color flow cytometric contour plots showing the correlated expression of CD19 versus TCL1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Right Panel - Immunohistofluorescent staining of TCL1 in human tonsil. Following antigen retrieval with BD Pharmingen Retrievagen A Buffer Cat. No. 550524 , a formalin-fixed, paraffin-embedded human tonsil section was blocked using an Avidin/Biotin Blocking Kit Vector Laboratories, Cat. No. SP-2001 , then stained with Alexa Fluor 647 Mouse Anti-Human TCL1 Cat. No.565856 ; pseudocolored red and BD Horizon BV421 Mouse Anti-Human CD45 Cat. No. 563879/563880; pseudocolored green antibodies. Nuclear or cytoplasmic TCL1 staining was detected in some cells. Images were acquired and merged using an epifluorescence microscopic system. Original magnification, 20 . Analyses of TCL1 expression Left Panel - Two-color flow cytometric analysis of TCL1 expression in human peripheral blood lymphocytes PBL . Human peripheral blood mononuclear cells were stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441 . The cells were washed, fixed, and permeabilized with the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . Cells were then stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; Left Plot or Alexa Fluor 647 Mouse Anti-Human TCL1 antibody Cat. No. 565856; Right Plot . Two-color flow cytometric contour plots showing the correlated expression of CD19 versus TCL1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Right Panel - Immunohistofluorescent staining of TCL1 in human tonsil. Following antigen retrieval with BD Pharmingen Retrievagen A Buffer Cat. No. 550524 , a formalin-fixed, paraffin-embedded human tonsil section was blocked using an Avidin/Biotin Blocking Kit Vector Laboratories, Cat. No. SP-2001 , then stained with Alexa Fluor 647 Mouse Anti-Human TCL1 Cat. No.565856 ; pseudocolored red and BD Horizon BV421 Mouse Anti-Human CD45 Cat. No. 563879/563880; pseudocolored green antibodies. Nuclear or cytoplasmic TCL1 staining was detected in some cells. Images were acquired and merged using an epifluorescence microscopic system. Original magnification, 20 . Show More Analyses of TCL1 expression Left Panel - Two-color flow cytometric analysis of TCL1 expression in human peripheral blood lymphocytes PBL . Human peripheral blood mononuclear cells were stained with BD Horizon BV421 Mouse Anti-Human CD19 antibody Cat. No. 562440/562441 . The cells were washed, fixed, and permeabilized with the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . Cells were then stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 565378; Left Plot or Alexa Fluor 647 Mouse Anti-Human TCL1 antibody Cat. No. 565856; Right Plot . Two-color flow cytometric contour plots showing the correlated expression of CD19 versus TCL1 or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Right Panel - Immunohistofluorescent staining of TCL1 in human tonsil. Following antigen retrieval with BD Pharmingen Retrievagen A Buffer Cat. No. 550524 , a formalin-fixed, paraffin-embedded human tonsil section was blocked using an Avidin/Biotin Blocking Kit Vector Laboratories, Cat. No. SP-2001 , then stained with Alexa Fluor 647 Mouse Anti-Human TCL1 Cat. No.565856 ; pseudocolored red and BD Horizon BV421 Mouse Anti-Human CD45 Cat. No. 563879/563880; pseudocolored green antibodies. Nuclear or cytoplasmic TCL1 staining was detected in some cells. Images were acquired and merged using an epifluorescence microscopic system. Original magnification, 20 .