BD PharmingenTM Alexa Fluor 647 Mouse Anti-Human VHL

Description

Flow cytometric analysis of VHL expression in VHL-transfected 293F cells. Untransfected dashed line histogram and human VHL-transfected solid line histogram 293F cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with Alexa Fluor 647 Mouse Anti-Human VHL antibody Cat. No. 564184 using BD Biosciences Protocol for Intracellular Staining. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Flow cytometric analysis of VHL expression in VHL-transfected 293F cells. Untransfected dashed line histogram and human VHL-transfected solid line histogram 293F cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with Alexa Fluor 647 Mouse Anti-Human VHL antibody Cat. No. 564184 using BD Biosciences Protocol for Intracellular Staining. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Show More Flow cytometric analysis of VHL expression in VHL-transfected 293F cells. Untransfected dashed line histogram and human VHL-transfected solid line histogram 293F cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were then washed and stained with Alexa Fluor 647 Mouse Anti-Human VHL antibody Cat. No. 564184 using BD Biosciences Protocol for Intracellular Staining. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System.