BD PharmingenTM Alexa Fluor 647 Mouse anti-MAP2B

Description

Analysis of MAP2B in H9 cells. H9 human embryonic stem ES cells WiCell, Madison, WI were differentiated into Neural Precursor cells NPCs and grown for 4 passages before differentiating into neurons and glia for 12 days. The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 20 minutes at room temperature, permeabilized with BD Phosflow Perm Buffer I Cat. No. 557885 , and then stained with either Alexa Fluor 647 Mouse anti-MAP2B solid line or Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557732, dashed line . This antibody also works in BD PhosFlow Perm Buffer II or III Cat. No. 558052 or 558050, respectively . Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Analysis of MAP2B in H9 cells. H9 human embryonic stem ES cells WiCell, Madison, WI were differentiated into Neural Precursor cells NPCs and grown for 4 passages before differentiating into neurons and glia for 12 days. The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 20 minutes at room temperature, permeabilized with BD Phosflow Perm Buffer I Cat. No. 557885 , and then stained with either Alexa Fluor 647 Mouse anti-MAP2B solid line or Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557732, dashed line . This antibody also works in BD PhosFlow Perm Buffer II or III Cat. No. 558052 or 558050, respectively . Flow cytometry was performed on a BD FACSCanto II flow cytometry system. Show More Analysis of MAP2B in H9 cells. H9 human embryonic stem ES cells WiCell, Madison, WI were differentiated into Neural Precursor cells NPCs and grown for 4 passages before differentiating into neurons and glia for 12 days. The cells were fixed BD Cytofix buffer, Cat. No. 554655 for 20 minutes at room temperature, permeabilized with BD Phosflow Perm Buffer I Cat. No. 557885 , and then stained with either Alexa Fluor 647 Mouse anti-MAP2B solid line or Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557732, dashed line . This antibody also works in BD PhosFlow Perm Buffer II or III Cat. No. 558052 or 558050, respectively . Flow cytometry was performed on a BD FACSCanto II flow cytometry system.