BD PharmingenTM Alexa Fluor 647 Mouse Anti-Mouse CD207 (Langerin)

Description

Multicolor flow cytometric analysis of mouse CD207 Langerin expression on mouse spleen cells. Mouse spleen cells were prepared using collagenase treatment followed by washing. The cells were stained with BD Horizon Fixable Viability Stain 450 Cat. No. 562247 , and then fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were stained with PE Hamster Anti-Mouse CD11c Cat. No. 553802/557401/561044 , BD Horizon PE-CF594 Rat Anti-Mouse CD49b Cat. No. 562453 , FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088/553087/561877 , Alexa Fluor 700 Rat Anti-Mouse Ly-6G/6C Cat. No. 557979 , and PerCP-Cy5.5 Hamster Anti-Mouse CD3e Cat. No. 551163/561108 antibodies and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; Left Panel or Mouse Anti-Mouse CD207 antibody Cat. No. 564937; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD11c versus CD207 Langerin or Ig Isotype control staining were derived from Fixable Viability Stain 450-negative and CD49b- CD45R/B220- Ly6G/6C- CD3- gated events with the forward and side-scatter light characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of mouse CD207 Langerin expression on mouse spleen cells. Mouse spleen cells were prepared using collagenase treatment followed by washing. The cells were stained with BD Horizon Fixable Viability Stain 450 Cat. No. 562247 , and then fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were stained with PE Hamster Anti-Mouse CD11c Cat. No. 553802/557401/561044 , BD Horizon PE-CF594 Rat Anti-Mouse CD49b Cat. No. 562453 , FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088/553087/561877 , Alexa Fluor 700 Rat Anti-Mouse Ly-6G/6C Cat. No. 557979 , and PerCP-Cy5.5 Hamster Anti-Mouse CD3e Cat. No. 551163/561108 antibodies and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; Left Panel or Mouse Anti-Mouse CD207 antibody Cat. No. 564937; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD11c versus CD207 Langerin or Ig Isotype control staining were derived from Fixable Viability Stain 450-negative and CD49b- CD45R/B220- Ly6G/6C- CD3- gated events with the forward and side-scatter light characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of mouse CD207 Langerin expression on mouse spleen cells. Mouse spleen cells were prepared using collagenase treatment followed by washing. The cells were stained with BD Horizon Fixable Viability Stain 450 Cat. No. 562247 , and then fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were stained with PE Hamster Anti-Mouse CD11c Cat. No. 553802/557401/561044 , BD Horizon PE-CF594 Rat Anti-Mouse CD49b Cat. No. 562453 , FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088/553087/561877 , Alexa Fluor 700 Rat Anti-Mouse Ly-6G/6C Cat. No. 557979 , and PerCP-Cy5.5 Hamster Anti-Mouse CD3e Cat. No. 551163/561108 antibodies and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; Left Panel or Mouse Anti-Mouse CD207 antibody Cat. No. 564937; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD11c versus CD207 Langerin or Ig Isotype control staining were derived from Fixable Viability Stain 450-negative and CD49b- CD45R/B220- Ly6G/6C- CD3- gated events with the forward and side-scatter light characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of mouse CD207 Langerin expression on mouse spleen cells. Mouse spleen cells were prepared using collagenase treatment followed by washing. The cells were stained with BD Horizon Fixable Viability Stain 450 Cat. No. 562247 , and then fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were stained with PE Hamster Anti-Mouse CD11c Cat. No. 553802/557401/561044 , BD Horizon PE-CF594 Rat Anti-Mouse CD49b Cat. No. 562453 , FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088/553087/561877 , Alexa Fluor 700 Rat Anti-Mouse Ly-6G/6C Cat. No. 557979 , and PerCP-Cy5.5 Hamster Anti-Mouse CD3e Cat. No. 551163/561108 antibodies and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; Left Panel or Mouse Anti-Mouse CD207 antibody Cat. No. 564937; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD11c versus CD207 Langerin or Ig Isotype control staining were derived from Fixable Viability Stain 450-negative and CD49b- CD45R/B220- Ly6G/6C- CD3- gated events with the forward and side-scatter light characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of mouse CD207 Langerin expression on mouse spleen cells. Mouse spleen cells were prepared using collagenase treatment followed by washing. The cells were stained with BD Horizon Fixable Viability Stain 450 Cat. No. 562247 , and then fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were stained with PE Hamster Anti-Mouse CD11c Cat. No. 553802/557401/561044 , BD Horizon PE-CF594 Rat Anti-Mouse CD49b Cat. No. 562453 , FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088/553087/561877 , Alexa Fluor 700 Rat Anti-Mouse Ly-6G/6C Cat. No. 557979 , and PerCP-Cy5.5 Hamster Anti-Mouse CD3e Cat. No. 551163/561108 antibodies and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; Left Panel or Mouse Anti-Mouse CD207 antibody Cat. No. 564937; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD11c versus CD207 Langerin or Ig Isotype control staining were derived from Fixable Viability Stain 450-negative and CD49b- CD45R/B220- Ly6G/6C- CD3- gated events with the forward and side-scatter light characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of mouse CD207 Langerin expression on mouse spleen cells. Mouse spleen cells were prepared using collagenase treatment followed by washing. The cells were stained with BD Horizon Fixable Viability Stain 450 Cat. No. 562247 , and then fixed and permeabilized using the BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit Cat. No. 554714 . The cells were stained with PE Hamster Anti-Mouse CD11c Cat. No. 553802/557401/561044 , BD Horizon PE-CF594 Rat Anti-Mouse CD49b Cat. No. 562453 , FITC Rat Anti-Mouse CD45R/B220 Cat. No. 553088/553087/561877 , Alexa Fluor 700 Rat Anti-Mouse Ly-6G/6C Cat. No. 557979 , and PerCP-Cy5.5 Hamster Anti-Mouse CD3e Cat. No. 551163/561108 antibodies and either Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557714; Left Panel or Mouse Anti-Mouse CD207 antibody Cat. No. 564937; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD11c versus CD207 Langerin or Ig Isotype control staining were derived from Fixable Viability Stain 450-negative and CD49b- CD45R/B220- Ly6G/6C- CD3- gated events with the forward and side-scatter light characteristics of intact cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.