BD PharmingenTM Alexa Fluor 647 Mouse anti-NeuroD1

Description

Flow cytometric analysis of NeuroD1 expression on human retinoblastoma. Y79 cells ATCC, HTB-18 were fixed with BD Cytofix fixation buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . The cells were stained with either Alexa Fluor 647 Mouse IgG1, kappa isotype control dashed line, Cat. No. 557732 or Alexa Fluor 647 Mouse anti-NeuroD1 monoclonal antibody at matched concentrations. Flow cytometry was performed on a BD CantoII flow cytometry system. We do not recommend BD Phosflow Perm/Wash Buffer I for permeabilization. Flow cytometric analysis of NeuroD1 expression on human retinoblastoma. Y79 cells ATCC, HTB-18 were fixed with BD Cytofix fixation buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . The cells were stained with either Alexa Fluor 647 Mouse IgG1, kappa isotype control dashed line, Cat. No. 557732 or Alexa Fluor 647 Mouse anti-NeuroD1 monoclonal antibody at matched concentrations. Flow cytometry was performed on a BD CantoII flow cytometry system. We do not recommend BD Phosflow Perm/Wash Buffer I for permeabilization. Show More Flow cytometric analysis of NeuroD1 expression on human retinoblastoma. Y79 cells ATCC, HTB-18 were fixed with BD Cytofix fixation buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm buffer III Cat. No. 558050 . The cells were stained with either Alexa Fluor 647 Mouse IgG1, kappa isotype control dashed line, Cat. No. 557732 or Alexa Fluor 647 Mouse anti-NeuroD1 monoclonal antibody at matched concentrations. Flow cytometry was performed on a BD CantoII flow cytometry system. We do not recommend BD Phosflow Perm/Wash Buffer I for permeabilization.