BD PharmingenTM Alexa Fluor 647 Mouse Anti-Nkx2.2

Description

Flow cytometric analysis of Nkx2.2 expression in transfected human embryonic kidney cells. Nkx2.2-transfected 293-F cells were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574 and stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 557903, dashed line or Alexa Fluor 647 Mouse Anti-Nkx2.2 solid line . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact 293-F cells. Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System. Immunofluorescent staining of Nkx2.2 transfected human embryonic kidney cells. Nkx2.2-transfected 293-F cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and stained with Alexa Fluor 647 Cross-Reactive Anti-Nkx2.2 antibody pseudo-colored red at 2.5 mug/mL. The nuclear counter-staining was with DAPI pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision software. Permeabilization with BD Perm/Wash Buffer Cat No. 554723 or 0.1 % Triton X-100 buffer is also suitable for use with this antibody. Flow cytometric analysis of Nkx2.2 expression in human embryonic kidney cells. 293-F cells Life Technologies R790-07 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 557903, dashed line or Alexa Fluor 647 Mouse Anti-Nkx2.2 solid line . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact 293-F cells. Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System. Flow cytometric analysis of Nkx2.2 expression in transfected human embryonic kidney cells. Nkx2.2-transfected 293-F cells were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574 and stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 557903, dashed line or Alexa Fluor 647 Mouse Anti-Nkx2.2 solid line . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact 293-F cells. Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System. Show More Immunofluorescent staining of Nkx2.2 transfected human embryonic kidney cells. Nkx2.2-transfected 293-F cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and stained with Alexa Fluor 647 Cross-Reactive Anti-Nkx2.2 antibody pseudo-colored red at 2.5 mug/mL. The nuclear counter-staining was with DAPI pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision software. Permeabilization with BD Perm/Wash Buffer Cat No. 554723 or 0.1 % Triton X-100 buffer is also suitable for use with this antibody. Show More Flow cytometric analysis of Nkx2.2 expression in human embryonic kidney cells. 293-F cells Life Technologies R790-07 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 557903, dashed line or Alexa Fluor 647 Mouse Anti-Nkx2.2 solid line . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact 293-F cells. Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System. Show More Flow cytometric analysis of Nkx2.2 expression in transfected human embryonic kidney cells. Nkx2.2-transfected 293-F cells were fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574 and stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 557903, dashed line or Alexa Fluor 647 Mouse Anti-Nkx2.2 solid line . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact 293-F cells. Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System. Immunofluorescent staining of Nkx2.2 transfected human embryonic kidney cells. Nkx2.2-transfected 293-F cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and stained with Alexa Fluor 647 Cross-Reactive Anti-Nkx2.2 antibody pseudo-colored red at 2.5 mug/mL. The nuclear counter-staining was with DAPI pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision software. Permeabilization with BD Perm/Wash Buffer Cat No. 554723 or 0.1 % Triton X-100 buffer is also suitable for use with this antibody. Flow cytometric analysis of Nkx2.2 expression in human embryonic kidney cells. 293-F cells Life Technologies R790-07 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 , and stained with either Alexa Fluor 647 Mouse IgG2b, kappa Isotype Control Cat. No. 557903, dashed line or Alexa Fluor 647 Mouse Anti-Nkx2.2 solid line . The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact 293-F cells. Flow cytometry was performed using a BD FACSCanto II Flow Cytometer System.