BD PharmingenTM Alexa Fluor 647 Mouse Anti-Nur77

Description

Flow cytometric analysis of upregulated Nur77 expression in stimulated mouse thymocytes. Thymocytes from C57BL/6 mice were left unstimulated gray line histogram or stimulated dashed and solid black line histograms with Phorbol 12-Myristate 13-Acetate PMA; 20 ng/ml and Ionomycin 500 ng/ml for 2 hours. The cells were then stained intracellularly with Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; black dashed line histogram or Alexa Fluor 647 Mouse Anti-Nur77 antibody Cat. No. 566735; gray line or solid black line histogram at 0.125 mug/test using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . Histograms showing Nur77 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of upregulated Nur77 expression in stimulated mouse thymocytes. Thymocytes from C57BL/6 mice were left unstimulated gray line histogram or stimulated dashed and solid black line histograms with Phorbol 12-Myristate 13-Acetate PMA; 20 ng/ml and Ionomycin 500 ng/ml for 2 hours. The cells were then stained intracellularly with Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; black dashed line histogram or Alexa Fluor 647 Mouse Anti-Nur77 antibody Cat. No. 566735; gray line or solid black line histogram at 0.125 mug/test using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . Histograms showing Nur77 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of upregulated Nur77 expression in stimulated mouse thymocytes. Thymocytes from C57BL/6 mice were left unstimulated gray line histogram or stimulated dashed and solid black line histograms with Phorbol 12-Myristate 13-Acetate PMA; 20 ng/ml and Ionomycin 500 ng/ml for 2 hours. The cells were then stained intracellularly with Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557732; black dashed line histogram or Alexa Fluor 647 Mouse Anti-Nur77 antibody Cat. No. 566735; gray line or solid black line histogram at 0.125 mug/test using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . Histograms showing Nur77 expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.