BD PharmingenTM Alexa Fluor 647 Mouse anti-Oct3/4

Description

Flow cytometric analysis of Alexa Fluor 647 anti-Oct3/4 in H9 cells. H9 human embryonic stem ES cells WiCell, Madison, WI were grown on BD Matrigel hESC-qualified matrix Cat. No. 354277 . Cells were dissociated into a single cell suspension and fixed BD Cytofix buffer, Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , and then stained with either Alexa Fluor 647 Mouse anti-Oct3/4 solid line or Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557783, dashed line . Flow cytometry was performed on a BD FACSCanto II flow cytometry system. This antibody also works in BD Phosflow Perm Buffer II and III. Flow cytometric analysis of Alexa Fluor 647 anti-Oct3/4 in H9 cells. H9 human embryonic stem ES cells WiCell, Madison, WI were grown on BD Matrigel hESC-qualified matrix Cat. No. 354277 . Cells were dissociated into a single cell suspension and fixed BD Cytofix buffer, Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , and then stained with either Alexa Fluor 647 Mouse anti-Oct3/4 solid line or Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557783, dashed line . Flow cytometry was performed on a BD FACSCanto II flow cytometry system. This antibody also works in BD Phosflow Perm Buffer II and III. Show More Flow cytometric analysis of Alexa Fluor 647 anti-Oct3/4 in H9 cells. H9 human embryonic stem ES cells WiCell, Madison, WI were grown on BD Matrigel hESC-qualified matrix Cat. No. 354277 . Cells were dissociated into a single cell suspension and fixed BD Cytofix buffer, Cat. No. 554655 , permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , and then stained with either Alexa Fluor 647 Mouse anti-Oct3/4 solid line or Alexa Fluor 647 Mouse IgG1, kappa Isotype Control Cat. No. 557783, dashed line . Flow cytometry was performed on a BD FACSCanto II flow cytometry system. This antibody also works in BD Phosflow Perm Buffer II and III.