

561476 ·
BDB Bioscience · Cat: 561476
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Description
Multicolor flow cytometric analysis of CD3 expression on pig peripheral blood lymphocytes. Pig whole blood was stained simultaneously with Alexa Fluor 647 Mouse Anti-Pig CD3epsilon antibody Cat. No. 561476 and PE-Cy7 Mouse Anti-Pig CD4 antibody Cat. No. 561473 . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . A two-color flow cytometric dot plot showing the correlated expression of CD3 versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD3 expression on pig peripheral blood lymphocytes. Pig whole blood was stained simultaneously with Alexa Fluor 647 Mouse Anti-Pig CD3epsilon antibody Cat. No. 561476 and PE-Cy7 Mouse Anti-Pig CD4 antibody Cat. No. 561473 . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . A two-color flow cytometric dot plot showing the correlated expression of CD3 versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD3 expression on pig peripheral blood lymphocytes. Pig whole blood was stained simultaneously with Alexa Fluor 647 Mouse Anti-Pig CD3epsilon antibody Cat. No. 561476 and PE-Cy7 Mouse Anti-Pig CD4 antibody Cat. No. 561473 . The erythrocytes were lysed with BD PharmLyse Lysing Buffer Cat. No. 555899 . A two-color flow cytometric dot plot showing the correlated expression of CD3 versus CD4 was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.







