BD PharmingenTM Alexa Fluor 647 Mouse Anti-PLZF

Description

Two color flow cytometric analysis of PLZF expression by mouse thymocytes and human peripheral blood mononuclear cells. Left Panel: Human peripheral blood mononuclear cells PBMC were fixed and permeabilized using BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were subsequently washed with BD Perm/Wash Buffer Cat. No. 554723 and stained with PE Mouse Anti-Human NCAM-1 CD56 Cat. No. 563238 and Alexa Fluor 647 Mouse Anti-PLZF antibodies. The two-color flow cytometric contour plots shows the coexpression of CD56 versus PLZF for gated events with the light-scatter characteristics of intact lymphocytes. Right Panel: BALB/c mouse thymocytes were stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 . The cells were washed and then fixed and permeabilized using BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were washed with BD Perm/Wash Buffer Cat. No. 554723 and stained with Alexa Fluor 647 Mouse Anti-PLZF antibody or matched isotype control Cat. No. 557783/557732 . The two-color flow cytometric contour plots shows the correlated expression patterns of CD3e versus PLZF for gated events with the forward and side light-scatter characteristics of intact thymocytes. Flow cytometric analyses were performed using a BD LSRFortessa Cell Analyzer System. Two color flow cytometric analysis of PLZF expression by mouse thymocytes and human peripheral blood mononuclear cells. Left Panel: Human peripheral blood mononuclear cells PBMC were fixed and permeabilized using BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were subsequently washed with BD Perm/Wash Buffer Cat. No. 554723 and stained with PE Mouse Anti-Human NCAM-1 CD56 Cat. No. 563238 and Alexa Fluor 647 Mouse Anti-PLZF antibodies. The two-color flow cytometric contour plots shows the coexpression of CD56 versus PLZF for gated events with the light-scatter characteristics of intact lymphocytes. Right Panel: BALB/c mouse thymocytes were stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 . The cells were washed and then fixed and permeabilized using BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were washed with BD Perm/Wash Buffer Cat. No. 554723 and stained with Alexa Fluor 647 Mouse Anti-PLZF antibody or matched isotype control Cat. No. 557783/557732 . The two-color flow cytometric contour plots shows the correlated expression patterns of CD3e versus PLZF for gated events with the forward and side light-scatter characteristics of intact thymocytes. Flow cytometric analyses were performed using a BD LSRFortessa Cell Analyzer System. Show More Two color flow cytometric analysis of PLZF expression by mouse thymocytes and human peripheral blood mononuclear cells. Left Panel: Human peripheral blood mononuclear cells PBMC were fixed and permeabilized using BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were subsequently washed with BD Perm/Wash Buffer Cat. No. 554723 and stained with PE Mouse Anti-Human NCAM-1 CD56 Cat. No. 563238 and Alexa Fluor 647 Mouse Anti-PLZF antibodies. The two-color flow cytometric contour plots shows the coexpression of CD56 versus PLZF for gated events with the light-scatter characteristics of intact lymphocytes. Right Panel: BALB/c mouse thymocytes were stained with FITC Hamster Anti-Mouse CD3e antibody Cat. No. 553061/553062/561827 . The cells were washed and then fixed and permeabilized using BD Cytofix/Cytoperm Fixation and Permeabilization Solution Cat. No. 554722 . The cells were washed with BD Perm/Wash Buffer Cat. No. 554723 and stained with Alexa Fluor 647 Mouse Anti-PLZF antibody or matched isotype control Cat. No. 557783/557732 . The two-color flow cytometric contour plots shows the correlated expression patterns of CD3e versus PLZF for gated events with the forward and side light-scatter characteristics of intact thymocytes. Flow cytometric analyses were performed using a BD LSRFortessa Cell Analyzer System.