BD PharmingenTM Alexa Fluor 647 Mouse Anti-Rat Integrin alphaE2 (CD103)

Description

Multiparameter flow cytometric analysis of Integrin alphaE2 CD103 expression on GM-CSF stimulated splenic leucocytes. Lewis rat Splenocytes were cultured overnight with Recombinant Rat GM-CSF Cat. No. 555111 . The cells were harvested and then pre-incubated with Purified Mouse Anti-Rat CD32 Cat. No. 550270/550271 . The cells were then stained with either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557714, left panel or Alexa Fluor 647 Mouse Anti-Rat Integrin alphaE2 CD103 Cat. No. 565286; right panel . Two-parameter flow cytometric contour plots showing the correlated expression of Integrin alphaE2 CD103 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side-light scattering characteristics of viable leucocytes. Flow cytometric analysis was performed on a BD LSR II. Multiparameter flow cytometric analysis of Integrin alphaE2 CD103 expression on GM-CSF stimulated splenic leucocytes. Lewis rat Splenocytes were cultured overnight with Recombinant Rat GM-CSF Cat. No. 555111 . The cells were harvested and then pre-incubated with Purified Mouse Anti-Rat CD32 Cat. No. 550270/550271 . The cells were then stained with either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557714, left panel or Alexa Fluor 647 Mouse Anti-Rat Integrin alphaE2 CD103 Cat. No. 565286; right panel . Two-parameter flow cytometric contour plots showing the correlated expression of Integrin alphaE2 CD103 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side-light scattering characteristics of viable leucocytes. Flow cytometric analysis was performed on a BD LSR II. Multiparameter flow cytometric analysis of Integrin alphaE2 CD103 expression on GM-CSF stimulated splenic leucocytes. Lewis rat Splenocytes were cultured overnight with Recombinant Rat GM-CSF Cat. No. 555111 . The cells were harvested and then pre-incubated with Purified Mouse Anti-Rat CD32 Cat. No. 550270/550271 . The cells were then stained with either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557714, left panel or Alexa Fluor 647 Mouse Anti-Rat Integrin alphaE2 CD103 Cat. No. 565286; right panel . Two-parameter flow cytometric contour plots showing the correlated expression of Integrin alphaE2 CD103 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side-light scattering characteristics of viable leucocytes. Flow cytometric analysis was performed on a BD LSR II. Show More Multiparameter flow cytometric analysis of Integrin alphaE2 CD103 expression on GM-CSF stimulated splenic leucocytes. Lewis rat Splenocytes were cultured overnight with Recombinant Rat GM-CSF Cat. No. 555111 . The cells were harvested and then pre-incubated with Purified Mouse Anti-Rat CD32 Cat. No. 550270/550271 . The cells were then stained with either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557714, left panel or Alexa Fluor 647 Mouse Anti-Rat Integrin alphaE2 CD103 Cat. No. 565286; right panel . Two-parameter flow cytometric contour plots showing the correlated expression of Integrin alphaE2 CD103 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side-light scattering characteristics of viable leucocytes. Flow cytometric analysis was performed on a BD LSR II. Show More Multiparameter flow cytometric analysis of Integrin alphaE2 CD103 expression on GM-CSF stimulated splenic leucocytes. Lewis rat Splenocytes were cultured overnight with Recombinant Rat GM-CSF Cat. No. 555111 . The cells were harvested and then pre-incubated with Purified Mouse Anti-Rat CD32 Cat. No. 550270/550271 . The cells were then stained with either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557714, left panel or Alexa Fluor 647 Mouse Anti-Rat Integrin alphaE2 CD103 Cat. No. 565286; right panel . Two-parameter flow cytometric contour plots showing the correlated expression of Integrin alphaE2 CD103 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side-light scattering characteristics of viable leucocytes. Flow cytometric analysis was performed on a BD LSR II. Multiparameter flow cytometric analysis of Integrin alphaE2 CD103 expression on GM-CSF stimulated splenic leucocytes. Lewis rat Splenocytes were cultured overnight with Recombinant Rat GM-CSF Cat. No. 555111 . The cells were harvested and then pre-incubated with Purified Mouse Anti-Rat CD32 Cat. No. 550270/550271 . The cells were then stained with either Alexa Fluor 647 Mouse IgG1 kappa Isotype Control Cat. No. 557714, left panel or Alexa Fluor 647 Mouse Anti-Rat Integrin alphaE2 CD103 Cat. No. 565286; right panel . Two-parameter flow cytometric contour plots showing the correlated expression of Integrin alphaE2 CD103 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side-light scattering characteristics of viable leucocytes. Flow cytometric analysis was performed on a BD LSR II.