BD PharmingenTM Alexa Fluor 647 Mouse Anti-Sox2

Description

Flow cytometric analysis of Sox2 on human embryonic stem ES cells. H7 human ES cells WiCell, Madison, WI passage 46 grown on irradiated mouse embryonic fibroblasts were harvested with Accutase Cat No. 561527 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . Cells were permeablized with BD Phosflow Perm Buffer III Cat No. 558050 and stained with Alexa Fluor 647 Mouse anti-Human Sox2 antibody solid line or Alexa Fluor 647 mouse IgG1, kappa isotype control Clone MOPC-21, Cat. No. 557714, dashed line . Flow cytometry was performed on a BD LSR II flow cytometry system. Immunoflourescent staining of Sox2 on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI passage 39 grown in mTeSR1 medium StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . Cells were permeablized with BD Phosflow Perm Buffer III Cat No. 558050 and stained with Alexa Fluor 647 Mouse anti-Sox2 monoclonal antibody pseudo colored red at 5 mug/mL. Counter-staining of cell nuclei was with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Flow cytometric analysis of Sox2 on human embryonic stem ES cells. H7 human ES cells WiCell, Madison, WI passage 46 grown on irradiated mouse embryonic fibroblasts were harvested with Accutase Cat No. 561527 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . Cells were permeablized with BD Phosflow Perm Buffer III Cat No. 558050 and stained with Alexa Fluor 647 Mouse anti-Human Sox2 antibody solid line or Alexa Fluor 647 mouse IgG1, kappa isotype control Clone MOPC-21, Cat. No. 557714, dashed line . Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Immunoflourescent staining of Sox2 on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI passage 39 grown in mTeSR1 medium StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . Cells were permeablized with BD Phosflow Perm Buffer III Cat No. 558050 and stained with Alexa Fluor 647 Mouse anti-Sox2 monoclonal antibody pseudo colored red at 5 mug/mL. Counter-staining of cell nuclei was with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software. Show More Flow cytometric analysis of Sox2 on human embryonic stem ES cells. H7 human ES cells WiCell, Madison, WI passage 46 grown on irradiated mouse embryonic fibroblasts were harvested with Accutase Cat No. 561527 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . Cells were permeablized with BD Phosflow Perm Buffer III Cat No. 558050 and stained with Alexa Fluor 647 Mouse anti-Human Sox2 antibody solid line or Alexa Fluor 647 mouse IgG1, kappa isotype control Clone MOPC-21, Cat. No. 557714, dashed line . Flow cytometry was performed on a BD LSR II flow cytometry system. Immunoflourescent staining of Sox2 on human embryonic stem ES cells. H9 human ES cells WiCell, Madison, WI passage 39 grown in mTeSR1 medium StemCell Technologies on BD Matrigel hESC-qualified Matrix Cat. No. 354277 were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . Cells were permeablized with BD Phosflow Perm Buffer III Cat No. 558050 and stained with Alexa Fluor 647 Mouse anti-Sox2 monoclonal antibody pseudo colored red at 5 mug/mL. Counter-staining of cell nuclei was with Hoechst 33342 pseudo-colored blue . The images were captured on a BD Pathway 435 Cell Analyzer and merged using BD Attovision Software.