BD PharmingenTM Alexa Fluor 647 Mouse IgG1 kappa Isotype Control

Description

Expression of TNF by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 in the presence of Brefeldin A BD GolgiPlug Protein Transport Inhibitor, Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with PE Mouse Anti-Human CD8 Cat. No. 555367 and either Alexa Fluor647 Mouse Anti Human TNF Cat. No. 557733; left panel or Alexa Fluor 647 MOPC-21 immunoglobulin Cat. No. 557732, right panel . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Expression of TNF by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 in the presence of Brefeldin A BD GolgiPlug Protein Transport Inhibitor, Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with PE Mouse Anti-Human CD8 Cat. No. 555367 and either Alexa Fluor647 Mouse Anti Human TNF Cat. No. 557733; left panel or Alexa Fluor 647 MOPC-21 immunoglobulin Cat. No. 557732, right panel . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Expression of TNF by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 in the presence of Brefeldin A BD GolgiPlug Protein Transport Inhibitor, Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with PE Mouse Anti-Human CD8 Cat. No. 555367 and either Alexa Fluor647 Mouse Anti Human TNF Cat. No. 557733; left panel or Alexa Fluor 647 MOPC-21 immunoglobulin Cat. No. 557732, right panel . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Show More Expression of TNF by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 in the presence of Brefeldin A BD GolgiPlug Protein Transport Inhibitor, Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with PE Mouse Anti-Human CD8 Cat. No. 555367 and either Alexa Fluor647 Mouse Anti Human TNF Cat. No. 557733; left panel or Alexa Fluor 647 MOPC-21 immunoglobulin Cat. No. 557732, right panel . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Show More Expression of TNF by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 in the presence of Brefeldin A BD GolgiPlug Protein Transport Inhibitor, Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with PE Mouse Anti-Human CD8 Cat. No. 555367 and either Alexa Fluor647 Mouse Anti Human TNF Cat. No. 557733; left panel or Alexa Fluor 647 MOPC-21 immunoglobulin Cat. No. 557732, right panel . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Expression of TNF by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hours with PMA 5 ng/ml, Sigma, Cat. No. P-8139 in the presence of Brefeldin A BD GolgiPlug Protein Transport Inhibitor, Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with PE Mouse Anti-Human CD8 Cat. No. 555367 and either Alexa Fluor647 Mouse Anti Human TNF Cat. No. 557733; left panel or Alexa Fluor 647 MOPC-21 immunoglobulin Cat. No. 557732, right panel . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls.