BD PharmingenTM Alexa Fluor 647 Rat Anti-Blimp-1

Description

Multicolor flow cytometric analysis of Blimp-1 expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with BD Horizon BV786 Mouse Anti-Human CD27 Cat No. 563327 and BD Horizon BUV737 Mouse Anti-Human CD19 Cat. No. 564303 antibodies, washed, and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 . The cells were then stained with Alexa Fluor 647 Rat Anti-Blimp-1 antibody Cat. No. 565002 . Histograms Right Panel showing Blimp-1 expression were derived from either CD19+CD27- gray shaded histogram , CD19+CD27+ dashed line histogram , or CD19loCD27+ black solid line histogram gated events as indicated by the two-color contour plot Left Panel showing the correlated expression of CD27 versus CD19 from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of Blimp-1 expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with BD Horizon BV786 Mouse Anti-Human CD27 Cat No. 563327 and BD Horizon BUV737 Mouse Anti-Human CD19 Cat. No. 564303 antibodies, washed, and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 . The cells were then stained with Alexa Fluor 647 Rat Anti-Blimp-1 antibody Cat. No. 565002 . Histograms Right Panel showing Blimp-1 expression were derived from either CD19+CD27- gray shaded histogram , CD19+CD27+ dashed line histogram , or CD19loCD27+ black solid line histogram gated events as indicated by the two-color contour plot Left Panel showing the correlated expression of CD27 versus CD19 from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of Blimp-1 expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with BD Horizon BV786 Mouse Anti-Human CD27 Cat No. 563327 and BD Horizon BUV737 Mouse Anti-Human CD19 Cat. No. 564303 antibodies, washed, and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 . The cells were then stained with Alexa Fluor 647 Rat Anti-Blimp-1 antibody Cat. No. 565002 . Histograms Right Panel showing Blimp-1 expression were derived from either CD19+CD27- gray shaded histogram , CD19+CD27+ dashed line histogram , or CD19loCD27+ black solid line histogram gated events as indicated by the two-color contour plot Left Panel showing the correlated expression of CD27 versus CD19 from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of Blimp-1 expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with BD Horizon BV786 Mouse Anti-Human CD27 Cat No. 563327 and BD Horizon BUV737 Mouse Anti-Human CD19 Cat. No. 564303 antibodies, washed, and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 . The cells were then stained with Alexa Fluor 647 Rat Anti-Blimp-1 antibody Cat. No. 565002 . Histograms Right Panel showing Blimp-1 expression were derived from either CD19+CD27- gray shaded histogram , CD19+CD27+ dashed line histogram , or CD19loCD27+ black solid line histogram gated events as indicated by the two-color contour plot Left Panel showing the correlated expression of CD27 versus CD19 from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of Blimp-1 expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with BD Horizon BV786 Mouse Anti-Human CD27 Cat No. 563327 and BD Horizon BUV737 Mouse Anti-Human CD19 Cat. No. 564303 antibodies, washed, and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 . The cells were then stained with Alexa Fluor 647 Rat Anti-Blimp-1 antibody Cat. No. 565002 . Histograms Right Panel showing Blimp-1 expression were derived from either CD19+CD27- gray shaded histogram , CD19+CD27+ dashed line histogram , or CD19loCD27+ black solid line histogram gated events as indicated by the two-color contour plot Left Panel showing the correlated expression of CD27 versus CD19 from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of Blimp-1 expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stained with BD Horizon BV786 Mouse Anti-Human CD27 Cat No. 563327 and BD Horizon BUV737 Mouse Anti-Human CD19 Cat. No. 564303 antibodies, washed, and fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat No. 562574/562725 . The cells were then stained with Alexa Fluor 647 Rat Anti-Blimp-1 antibody Cat. No. 565002 . Histograms Right Panel showing Blimp-1 expression were derived from either CD19+CD27- gray shaded histogram , CD19+CD27+ dashed line histogram , or CD19loCD27+ black solid line histogram gated events as indicated by the two-color contour plot Left Panel showing the correlated expression of CD27 versus CD19 from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.