BD PharmingenTM Alexa Fluor 647 Rat Anti-Mouse Blimp-1

Description

Two color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. BALB/c splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat.No. 553089/553090/561878 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat.No. 557690; Left Panel or Alexa Fluor 647 Rat Anti-Mouse Blimp-1 antibody Cat. No. 563643; Right Panel . The two-color flow cytometric contour plots show the correlated expression patterns of Blimp-1 or Ig Isotype control staining versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometry System. Two color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. BALB/c splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat.No. 553089/553090/561878 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat.No. 557690; Left Panel or Alexa Fluor 647 Rat Anti-Mouse Blimp-1 antibody Cat. No. 563643; Right Panel . The two-color flow cytometric contour plots show the correlated expression patterns of Blimp-1 or Ig Isotype control staining versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometry System. Two color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. BALB/c splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat.No. 553089/553090/561878 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat.No. 557690; Left Panel or Alexa Fluor 647 Rat Anti-Mouse Blimp-1 antibody Cat. No. 563643; Right Panel . The two-color flow cytometric contour plots show the correlated expression patterns of Blimp-1 or Ig Isotype control staining versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometry System. Show More Two color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. BALB/c splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat.No. 553089/553090/561878 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat.No. 557690; Left Panel or Alexa Fluor 647 Rat Anti-Mouse Blimp-1 antibody Cat. No. 563643; Right Panel . The two-color flow cytometric contour plots show the correlated expression patterns of Blimp-1 or Ig Isotype control staining versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometry System. Show More Two color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. BALB/c splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat.No. 553089/553090/561878 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat.No. 557690; Left Panel or Alexa Fluor 647 Rat Anti-Mouse Blimp-1 antibody Cat. No. 563643; Right Panel . The two-color flow cytometric contour plots show the correlated expression patterns of Blimp-1 or Ig Isotype control staining versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometry System. Two color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. BALB/c splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with PE Rat Anti-Mouse CD45R/B220 antibody Cat.No. 553089/553090/561878 . The cells were subsequently fixed and permeabilized with the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 and stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat.No. 557690; Left Panel or Alexa Fluor 647 Rat Anti-Mouse Blimp-1 antibody Cat. No. 563643; Right Panel . The two-color flow cytometric contour plots show the correlated expression patterns of Blimp-1 or Ig Isotype control staining versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells data not shown . Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometry System.