BD PharmingenTM Alexa Fluor 647 Rat Anti-Mouse CD177

Description

Two-color flow cytometric analysis of CD177 expression on mouse granulocytes. Bone marrow cells from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6C and Ly-6G Gr-1 antibody Cat. No. 562709 and either Alexa Fluor 647 Rat IgG2b, kappa Isotype Control Cat. No. 557691; Left Plot or Alexa Fluor 647 Rat Anti-Mouse CD177 antibody Cat. No. 566599; Right Plot at 0.5 mug/test. Two-color flow cytometric contour plots showing the correlated expression patterns of CD177 or Ig Isotype control staining versus Ly-6C and Ly-6G Gr-1 , were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD177 expression on mouse granulocytes. Bone marrow cells from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6C and Ly-6G Gr-1 antibody Cat. No. 562709 and either Alexa Fluor 647 Rat IgG2b, kappa Isotype Control Cat. No. 557691; Left Plot or Alexa Fluor 647 Rat Anti-Mouse CD177 antibody Cat. No. 566599; Right Plot at 0.5 mug/test. Two-color flow cytometric contour plots showing the correlated expression patterns of CD177 or Ig Isotype control staining versus Ly-6C and Ly-6G Gr-1 , were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD177 expression on mouse granulocytes. Bone marrow cells from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6C and Ly-6G Gr-1 antibody Cat. No. 562709 and either Alexa Fluor 647 Rat IgG2b, kappa Isotype Control Cat. No. 557691; Left Plot or Alexa Fluor 647 Rat Anti-Mouse CD177 antibody Cat. No. 566599; Right Plot at 0.5 mug/test. Two-color flow cytometric contour plots showing the correlated expression patterns of CD177 or Ig Isotype control staining versus Ly-6C and Ly-6G Gr-1 , were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD177 expression on mouse granulocytes. Bone marrow cells from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6C and Ly-6G Gr-1 antibody Cat. No. 562709 and either Alexa Fluor 647 Rat IgG2b, kappa Isotype Control Cat. No. 557691; Left Plot or Alexa Fluor 647 Rat Anti-Mouse CD177 antibody Cat. No. 566599; Right Plot at 0.5 mug/test. Two-color flow cytometric contour plots showing the correlated expression patterns of CD177 or Ig Isotype control staining versus Ly-6C and Ly-6G Gr-1 , were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Show More Two-color flow cytometric analysis of CD177 expression on mouse granulocytes. Bone marrow cells from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6C and Ly-6G Gr-1 antibody Cat. No. 562709 and either Alexa Fluor 647 Rat IgG2b, kappa Isotype Control Cat. No. 557691; Left Plot or Alexa Fluor 647 Rat Anti-Mouse CD177 antibody Cat. No. 566599; Right Plot at 0.5 mug/test. Two-color flow cytometric contour plots showing the correlated expression patterns of CD177 or Ig Isotype control staining versus Ly-6C and Ly-6G Gr-1 , were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific. Two-color flow cytometric analysis of CD177 expression on mouse granulocytes. Bone marrow cells from C57BL/6 mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon BV421 Rat Anti-Mouse Ly-6C and Ly-6G Gr-1 antibody Cat. No. 562709 and either Alexa Fluor 647 Rat IgG2b, kappa Isotype Control Cat. No. 557691; Left Plot or Alexa Fluor 647 Rat Anti-Mouse CD177 antibody Cat. No. 566599; Right Plot at 0.5 mug/test. Two-color flow cytometric contour plots showing the correlated expression patterns of CD177 or Ig Isotype control staining versus Ly-6C and Ly-6G Gr-1 , were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.