BD PharmingenTM Alexa Fluor 647 Rat Anti-Mouse CD19

Description

Immunohistofluorescent analysis of CD19 expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with Alexa Fluor 647 Rat Anti-Mouse CD19 antibody Cat. No. 557684, pseudo-colored green and Alexa Fluor 488 Rat Anti-Mouse CD8a antibody Cat. No. 557869, pseudo-colored red . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Two-color analysis of the expression of CD19 on mouse spleen B cells. C57BL/6 splenocytes were stained with PE Hamster Anti-Mouse CD3e Cat. No. 553063 and either Alexa Fluor 647 Rat IgG2a, kappa isotype control Cat. No. 557690, left panel or Alexa Fluor 647 Rat Anti-Mouse CD19 right panel . Nonviable leukocytes were excluded from the analysis by staining with Propodium Iodide Staining Solution Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur SE flow cytometry system. Immunohistofluorescent analysis of CD19 expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with Alexa Fluor 647 Rat Anti-Mouse CD19 antibody Cat. No. 557684, pseudo-colored green and Alexa Fluor 488 Rat Anti-Mouse CD8a antibody Cat. No. 557869, pseudo-colored red . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Show More Two-color analysis of the expression of CD19 on mouse spleen B cells. C57BL/6 splenocytes were stained with PE Hamster Anti-Mouse CD3e Cat. No. 553063 and either Alexa Fluor 647 Rat IgG2a, kappa isotype control Cat. No. 557690, left panel or Alexa Fluor 647 Rat Anti-Mouse CD19 right panel . Nonviable leukocytes were excluded from the analysis by staining with Propodium Iodide Staining Solution Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur SE flow cytometry system. Show More Immunohistofluorescent analysis of CD19 expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with Alexa Fluor 647 Rat Anti-Mouse CD19 antibody Cat. No. 557684, pseudo-colored green and Alexa Fluor 488 Rat Anti-Mouse CD8a antibody Cat. No. 557869, pseudo-colored red . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Two-color analysis of the expression of CD19 on mouse spleen B cells. C57BL/6 splenocytes were stained with PE Hamster Anti-Mouse CD3e Cat. No. 553063 and either Alexa Fluor 647 Rat IgG2a, kappa isotype control Cat. No. 557690, left panel or Alexa Fluor 647 Rat Anti-Mouse CD19 right panel . Nonviable leukocytes were excluded from the analysis by staining with Propodium Iodide Staining Solution Cat. No. 556463 . Flow cytometry was performed on a BD FACSCalibur SE flow cytometry system.