BD PharmingenTM Alexa Fluor 647 Rat Anti-Mouse CD206

Description

Two-color flow cytometric analysis of CD206 expression on mouse peritoneal exudate cells. Thioglycollate-elicited BALB/c mouse peritoneal exudate cells PEC were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The leucocytes were then stained with PE Rat Anti-Mouse CD107b Mac-3 antibody Cat. No. 553324 and either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 565250; Left Panel or Alexa Fluor 647 Rat Anti-Mouse CD206 antibody Cat. No. 565250; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD206 or Ig Isotype control staining versus CD107b were derived from gated events with the forward and side-light scattering characteristics of intact PEC. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Flow cytometric analysis of CD206 expression by J774A cells. Cells from the J774A Mouse macrophage, ATCC TIB-67 cell line were similarly fixed and permeabilized, stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control dashed line histogram or Alexa Fluor 647 Rat Anti-Mouse CD206 antibody solid line histogram , and analzyed by flow cytometry. The fluorescence histogram showing CD206 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact J774A cells. Two-color flow cytometric analysis of CD206 expression on mouse peritoneal exudate cells. Thioglycollate-elicited BALB/c mouse peritoneal exudate cells PEC were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The leucocytes were then stained with PE Rat Anti-Mouse CD107b Mac-3 antibody Cat. No. 553324 and either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 565250; Left Panel or Alexa Fluor 647 Rat Anti-Mouse CD206 antibody Cat. No. 565250; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD206 or Ig Isotype control staining versus CD107b were derived from gated events with the forward and side-light scattering characteristics of intact PEC. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Show More Flow cytometric analysis of CD206 expression by J774A cells. Cells from the J774A Mouse macrophage, ATCC TIB-67 cell line were similarly fixed and permeabilized, stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control dashed line histogram or Alexa Fluor 647 Rat Anti-Mouse CD206 antibody solid line histogram , and analzyed by flow cytometry. The fluorescence histogram showing CD206 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact J774A cells. Show More Two-color flow cytometric analysis of CD206 expression on mouse peritoneal exudate cells. Thioglycollate-elicited BALB/c mouse peritoneal exudate cells PEC were fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The leucocytes were then stained with PE Rat Anti-Mouse CD107b Mac-3 antibody Cat. No. 553324 and either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 565250; Left Panel or Alexa Fluor 647 Rat Anti-Mouse CD206 antibody Cat. No. 565250; Right Panel . Two-color flow cytometric contour plots showing the correlated expression of CD206 or Ig Isotype control staining versus CD107b were derived from gated events with the forward and side-light scattering characteristics of intact PEC. Flow cytometric analysis was performed using a BD FACSCanto II Flow Cytometer System. Flow cytometric analysis of CD206 expression by J774A cells. Cells from the J774A Mouse macrophage, ATCC TIB-67 cell line were similarly fixed and permeabilized, stained with either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control dashed line histogram or Alexa Fluor 647 Rat Anti-Mouse CD206 antibody solid line histogram , and analzyed by flow cytometry. The fluorescence histogram showing CD206 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact J774A cells.