BD PharmingenTM Alexa Fluor 647 Rat Anti-Mouse CD8a

Description

Immunohistofluorescent analysis of CD8a expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with Alexa Fluor 647 Rat-anti-Mouse CD8a Cat. No. 557682, pseudo-colored red , BD Horizon BV421 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 562922, pseudo-colored green , BD Horizon BV480 Rat Anti-Mouse CD4 antibody Cat. No. 565634, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Immunohistofluorescent analysis of CD8a expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with Alexa Fluor 647 Rat-anti-Mouse CD8a Cat. No. 557682, pseudo-colored red , BD Horizon BV421 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 562922, pseudo-colored green , BD Horizon BV480 Rat Anti-Mouse CD4 antibody Cat. No. 565634, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x. Show More Immunohistofluorescent analysis of CD8a expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection 5 mum was fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , blocked with 5 % goat serum and 1 % BSA diluted in 1x PBS, and stained with Alexa Fluor 647 Rat-anti-Mouse CD8a Cat. No. 557682, pseudo-colored red , BD Horizon BV421 Rat Anti-Mouse CD45R/B220 antibody Cat. No. 562922, pseudo-colored green , BD Horizon BV480 Rat Anti-Mouse CD4 antibody Cat. No. 565634, pseudo-colored blue . Images were captured on a standard epifluorescence microscope. Original magnification, 20x.