BD PharmingenTM Alexa Fluor 647 Rat Anti-Mouse ESAM

Description

Flow cytometric analysis of ESAM expression on bEnd.3 cells. Cells from the mouse bEnd.3 Brain Endothelioma, ATCC CRL-2299 cell line were stained with Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; dashed line histogram or Alexa Fluor 647 Mouse Anti-ESAM antibody Cat. No. 567162; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing ESAM expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of ESAM expression on bEnd.3 cells. Cells from the mouse bEnd.3 Brain Endothelioma, ATCC CRL-2299 cell line were stained with Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; dashed line histogram or Alexa Fluor 647 Mouse Anti-ESAM antibody Cat. No. 567162; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing ESAM expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of ESAM expression on bEnd.3 cells. Cells from the mouse bEnd.3 Brain Endothelioma, ATCC CRL-2299 cell line were stained with Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; dashed line histogram or Alexa Fluor 647 Mouse Anti-ESAM antibody Cat. No. 567162; solid line histogram at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing ESAM expression or Ig Isotype control staining was derived from gated events with the light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis were performed using a BD Celesta Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.