BD PharmingenTM Alexa Fluor 647 Rat Anti-Mouse Foxp3

Description

Multicolor flow cytometric analysis of Foxp3 expression in mouse splenic lymphocytes. BALB/c mouse splenic leucocytes were fixed and permeabilized using Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; Left Plot or Alexa Fluor 647 Rat Anti-Mouse Foxp3 antibody Cat. No. 563486; Right Plot at 0.06 mug/test. The bivariate pseudocolor density plots show the correlated expression patterns of CD4 versus Foxp3 or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa Cell Analyzer System and FloJo software. Data on this Technical Data Sheet are not lot-specific. Multicolor flow cytometric analysis of Foxp3 expression in mouse splenic lymphocytes. BALB/c mouse splenic leucocytes were fixed and permeabilized using Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; Left Plot or Alexa Fluor 647 Rat Anti-Mouse Foxp3 antibody Cat. No. 563486; Right Plot at 0.06 mug/test. The bivariate pseudocolor density plots show the correlated expression patterns of CD4 versus Foxp3 or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa Cell Analyzer System and FloJo software. Data on this Technical Data Sheet are not lot-specific. Multicolor flow cytometric analysis of Foxp3 expression in mouse splenic lymphocytes. BALB/c mouse splenic leucocytes were fixed and permeabilized using Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; Left Plot or Alexa Fluor 647 Rat Anti-Mouse Foxp3 antibody Cat. No. 563486; Right Plot at 0.06 mug/test. The bivariate pseudocolor density plots show the correlated expression patterns of CD4 versus Foxp3 or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa Cell Analyzer System and FloJo software. Data on this Technical Data Sheet are not lot-specific. Show More Multicolor flow cytometric analysis of Foxp3 expression in mouse splenic lymphocytes. BALB/c mouse splenic leucocytes were fixed and permeabilized using Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; Left Plot or Alexa Fluor 647 Rat Anti-Mouse Foxp3 antibody Cat. No. 563486; Right Plot at 0.06 mug/test. The bivariate pseudocolor density plots show the correlated expression patterns of CD4 versus Foxp3 or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa Cell Analyzer System and FloJo software. Data on this Technical Data Sheet are not lot-specific. Show More Multicolor flow cytometric analysis of Foxp3 expression in mouse splenic lymphocytes. BALB/c mouse splenic leucocytes were fixed and permeabilized using Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; Left Plot or Alexa Fluor 647 Rat Anti-Mouse Foxp3 antibody Cat. No. 563486; Right Plot at 0.06 mug/test. The bivariate pseudocolor density plots show the correlated expression patterns of CD4 versus Foxp3 or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa Cell Analyzer System and FloJo software. Data on this Technical Data Sheet are not lot-specific. Multicolor flow cytometric analysis of Foxp3 expression in mouse splenic lymphocytes. BALB/c mouse splenic leucocytes were fixed and permeabilized using Transcription Factor Buffer Set Cat. No. 562574/562725 . The cells were then stained with PE Rat Anti-Mouse CD4 Cat. No. 553048/553049/561837 either Alexa Fluor 647 Rat IgG2a, kappa Isotype Control Cat. No. 557690; Left Plot or Alexa Fluor 647 Rat Anti-Mouse Foxp3 antibody Cat. No. 563486; Right Plot at 0.06 mug/test. The bivariate pseudocolor density plots show the correlated expression patterns of CD4 versus Foxp3 or Ig Isotype control staining for gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis was performed using a BD LSRFortessa Cell Analyzer System and FloJo software. Data on this Technical Data Sheet are not lot-specific.