BD PharmingenTM Alexa Fluor 700 Mouse Anti-Human CD69

Description

Flow cytometric analysis for CD69 in stimulated peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for at least 4-6 hours with 20 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . Cells were then stained with either a Alexa Fluor 700 Mouse IgG1, kappa isotype control Cat. No. 557882; shaded histogram or with the Alexa Fluor 700 Mouse Anti-Human CD69 antibody Cat. No. 560739; unshaded histogram . Histograms were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Flow cytometric analysis for CD69 in stimulated peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for at least 4-6 hours with 20 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . Cells were then stained with either a Alexa Fluor 700 Mouse IgG1, kappa isotype control Cat. No. 557882; shaded histogram or with the Alexa Fluor 700 Mouse Anti-Human CD69 antibody Cat. No. 560739; unshaded histogram . Histograms were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system. Show More Flow cytometric analysis for CD69 in stimulated peripheral blood mononuclear cells PBMC . Human PBMC were stimulated for at least 4-6 hours with 20 ng/mL PMA Sigma-Aldrich Cat. No. P-8139 and 250 ng/mL calcium ionophore A23187 Sigma-Aldrich Cat. No. C-9275 . Cells were then stained with either a Alexa Fluor 700 Mouse IgG1, kappa isotype control Cat. No. 557882; shaded histogram or with the Alexa Fluor 700 Mouse Anti-Human CD69 antibody Cat. No. 560739; unshaded histogram . Histograms were derived from gated events based on light scattering characteristics for lymphocytes. Flow cytometry was performed on a BD LSR II flow cytometry system.