BD PharmingenTM Alexa Fluor 700 Mouse Anti-Human TNF

Description

Expression of TNF by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hrs with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of GolgiStop Cat. No. 554715 . Cells were harvested, fixed, permeabilized and stained with Alexa Fluor 700 Mouse Anti-Human TNF Cat. No. 557996 . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Expression of TNF by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hrs with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of GolgiStop Cat. No. 554715 . Cells were harvested, fixed, permeabilized and stained with Alexa Fluor 700 Mouse Anti-Human TNF Cat. No. 557996 . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls. Show More Expression of TNF by stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were stimulated for 4 hrs with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, P-8139 in the presence of GolgiStop Cat. No. 554715 . Cells were harvested, fixed, permeabilized and stained with Alexa Fluor 700 Mouse Anti-Human TNF Cat. No. 557996 . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plots were set based on the autofluorescence and isotype controls.