BD PharmingenTM Alexa Fluor 700 Rat Anti-Mouse CD4

Description

Multicolor flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and either Alexa Fluor 700 Rat IgG2a, kappa Isotype Control Cat. No. 557963; Left Plot or Alexa Fluor 700 Rat Anti-Mouse CD4 Cat. No. 557956/ 561025; Right Plot at 0.06 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3epsilon was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and either Alexa Fluor 700 Rat IgG2a, kappa Isotype Control Cat. No. 557963; Left Plot or Alexa Fluor 700 Rat Anti-Mouse CD4 Cat. No. 557956/ 561025; Right Plot at 0.06 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3epsilon was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD4 expression on mouse splenocytes. Mouse splenic leucocytes were stained with PE Hamster Anti-Mouse CD3e antibody Cat. No. 553063/553064/561824 and either Alexa Fluor 700 Rat IgG2a, kappa Isotype Control Cat. No. 557963; Left Plot or Alexa Fluor 700 Rat Anti-Mouse CD4 Cat. No. 557956/ 561025; Right Plot at 0.06 mug/test. The bivariate pseudocolor density plot showing the correlated expression of CD4 or Ig Isotype control staining versus CD3epsilon was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.