BD PharmingenTM Alexa Fluor 700 Rat Anti-Mouse CD8a

Description

Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with PE Rat Anti-Mouse CD3e Cat. No. 553063/553064/561824 and either Alexa Fluor 700 Rat IgG2a, kappa Isotype Control Cat. No. 557963; Left Plot or Alexa Fluor 700 Rat Anti-Mouse CD3e Cat. No. 557959; Right Plot antibodies. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with PE Rat Anti-Mouse CD3e Cat. No. 553063/553064/561824 and either Alexa Fluor 700 Rat IgG2a, kappa Isotype Control Cat. No. 557963; Left Plot or Alexa Fluor 700 Rat Anti-Mouse CD3e Cat. No. 557959; Right Plot antibodies. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with PE Rat Anti-Mouse CD3e Cat. No. 553063/553064/561824 and either Alexa Fluor 700 Rat IgG2a, kappa Isotype Control Cat. No. 557963; Left Plot or Alexa Fluor 700 Rat Anti-Mouse CD3e Cat. No. 557959; Right Plot antibodies. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with PE Rat Anti-Mouse CD3e Cat. No. 553063/553064/561824 and either Alexa Fluor 700 Rat IgG2a, kappa Isotype Control Cat. No. 557963; Left Plot or Alexa Fluor 700 Rat Anti-Mouse CD3e Cat. No. 557959; Right Plot antibodies. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with PE Rat Anti-Mouse CD3e Cat. No. 553063/553064/561824 and either Alexa Fluor 700 Rat IgG2a, kappa Isotype Control Cat. No. 557963; Left Plot or Alexa Fluor 700 Rat Anti-Mouse CD3e Cat. No. 557959; Right Plot antibodies. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multicolor flow cytometric analysis of CD8a expression on mouse splenocytes. Splenic leucocytes were stained with PE Rat Anti-Mouse CD3e Cat. No. 553063/553064/561824 and either Alexa Fluor 700 Rat IgG2a, kappa Isotype Control Cat. No. 557963; Left Plot or Alexa Fluor 700 Rat Anti-Mouse CD3e Cat. No. 557959; Right Plot antibodies. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot shows the correlated expression patterns of CD8a or Ig Isotype control staining versus CD3e for gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.