BD PharmingenTM Alexa Fluor 700 Rat anti-Mouse TNF

Description

Expression of TNF by stimulated BALB/c spleen cells. Splenocytes from BALB/c mice were stimulated for 4 hrs with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, Cat. No. I0634 in the presence of Brefeldin A GolgiPlug, Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with Alexa Fluor 700 Rat anti-Mouse TNF or Alexa Fluor 700 Rat IgG1 kappa Isotype Control data not shown . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plot was based on the autofluorescence and isotype controls. Expression of TNF by stimulated BALB/c spleen cells. Splenocytes from BALB/c mice were stimulated for 4 hrs with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, Cat. No. I0634 in the presence of Brefeldin A GolgiPlug, Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with Alexa Fluor 700 Rat anti-Mouse TNF or Alexa Fluor 700 Rat IgG1 kappa Isotype Control data not shown . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plot was based on the autofluorescence and isotype controls. Show More Expression of TNF by stimulated BALB/c spleen cells. Splenocytes from BALB/c mice were stimulated for 4 hrs with PMA 5 ng/ml, Sigma, Cat. No. P-8139 and Ionomycin 500 ng, Sigma, Cat. No. I0634 in the presence of Brefeldin A GolgiPlug, Cat. No. 555029 . Cells were harvested, fixed, permeabilized and stained with Alexa Fluor 700 Rat anti-Mouse TNF or Alexa Fluor 700 Rat IgG1 kappa Isotype Control data not shown . Dot plots were derived from gated events with the forward and side light scatter characteristics of lymphocytes. The quadrant markers for the bivariate dot plot was based on the autofluorescence and isotype controls.