BD PharmingenTM Alexa FluorTM 488 Mouse Anti-Biotin

Description

Multiparameter flow cytometric analysis of CD25 expression on viable Mouse splenic lymphocytes. Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and stained with Biotin Rat Anti-Mouse CD25 antibody Cat. No. 567831 . The cells were washed and then secondarily stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 567121; Left Plot or Alexa Fluor 488 Mouse Anti-Biotin antibody Cat. No. 570938/571010; Right Plot at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD25 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of CD25 expression on viable Mouse splenic lymphocytes. Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and stained with Biotin Rat Anti-Mouse CD25 antibody Cat. No. 567831 . The cells were washed and then secondarily stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 567121; Left Plot or Alexa Fluor 488 Mouse Anti-Biotin antibody Cat. No. 570938/571010; Right Plot at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD25 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of CD25 expression on viable Mouse splenic lymphocytes. Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and stained with Biotin Rat Anti-Mouse CD25 antibody Cat. No. 567831 . The cells were washed and then secondarily stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 567121; Left Plot or Alexa Fluor 488 Mouse Anti-Biotin antibody Cat. No. 570938/571010; Right Plot at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing CD25 expression or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.