BD PharmingenTM Alexa FluorTM 488 Mouse Anti-Human Active beta2 Integrin (CD18)

Description

Multiparameter flow cytometric analysis of Active beta2 Integrin CD18 expression on untreated or stimulated human peripheral blood leucocytes PBL collected with either heparin or EDTA anticoagulants. Left Panel: PBL collected with heparin were either untreated Top Plots or were stimulated with 200 nM PMA 37C, 20 min; Bottom Plots . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; Left Plots or Alexa Fluor 488 Mouse Anti-Human Active beta2 Integrin CD18 antibody Cat. No. 567943/567944; Right Plots . Erythrocytes were then lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plots showing the correlated expression of Active beta2 Integrin CD18 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Right Panel: PBL collected with the chelating agent, EDTA were similarly treated, stained, and analyzed by multiparameter flow cytometry. Multiparameter flow cytometric analysis of Active beta2 Integrin CD18 expression on untreated or stimulated human peripheral blood leucocytes PBL collected with either heparin or EDTA anticoagulants. Left Panel: PBL collected with heparin were either untreated Top Plots or were stimulated with 200 nM PMA 37C, 20 min; Bottom Plots . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; Left Plots or Alexa Fluor 488 Mouse Anti-Human Active beta2 Integrin CD18 antibody Cat. No. 567943/567944; Right Plots . Erythrocytes were then lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plots showing the correlated expression of Active beta2 Integrin CD18 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Right Panel: PBL collected with the chelating agent, EDTA were similarly treated, stained, and analyzed by multiparameter flow cytometry. Multiparameter flow cytometric analysis of Active beta2 Integrin CD18 expression on untreated or stimulated human peripheral blood leucocytes PBL collected with either heparin or EDTA anticoagulants. Left Panel: PBL collected with heparin were either untreated Top Plots or were stimulated with 200 nM PMA 37C, 20 min; Bottom Plots . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; Left Plots or Alexa Fluor 488 Mouse Anti-Human Active beta2 Integrin CD18 antibody Cat. No. 567943/567944; Right Plots . Erythrocytes were then lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plots showing the correlated expression of Active beta2 Integrin CD18 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Right Panel: PBL collected with the chelating agent, EDTA were similarly treated, stained, and analyzed by multiparameter flow cytometry. Show More Multiparameter flow cytometric analysis of Active beta2 Integrin CD18 expression on untreated or stimulated human peripheral blood leucocytes PBL collected with either heparin or EDTA anticoagulants. Left Panel: PBL collected with heparin were either untreated Top Plots or were stimulated with 200 nM PMA 37C, 20 min; Bottom Plots . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; Left Plots or Alexa Fluor 488 Mouse Anti-Human Active beta2 Integrin CD18 antibody Cat. No. 567943/567944; Right Plots . Erythrocytes were then lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plots showing the correlated expression of Active beta2 Integrin CD18 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Right Panel: PBL collected with the chelating agent, EDTA were similarly treated, stained, and analyzed by multiparameter flow cytometry. Show More Multiparameter flow cytometric analysis of Active beta2 Integrin CD18 expression on untreated or stimulated human peripheral blood leucocytes PBL collected with either heparin or EDTA anticoagulants. Left Panel: PBL collected with heparin were either untreated Top Plots or were stimulated with 200 nM PMA 37C, 20 min; Bottom Plots . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; Left Plots or Alexa Fluor 488 Mouse Anti-Human Active beta2 Integrin CD18 antibody Cat. No. 567943/567944; Right Plots . Erythrocytes were then lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plots showing the correlated expression of Active beta2 Integrin CD18 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Right Panel: PBL collected with the chelating agent, EDTA were similarly treated, stained, and analyzed by multiparameter flow cytometry. Multiparameter flow cytometric analysis of Active beta2 Integrin CD18 expression on untreated or stimulated human peripheral blood leucocytes PBL collected with either heparin or EDTA anticoagulants. Left Panel: PBL collected with heparin were either untreated Top Plots or were stimulated with 200 nM PMA 37C, 20 min; Bottom Plots . The cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; Left Plots or Alexa Fluor 488 Mouse Anti-Human Active beta2 Integrin CD18 antibody Cat. No. 567943/567944; Right Plots . Erythrocytes were then lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . The bivariate pseudocolor density plots showing the correlated expression of Active beta2 Integrin CD18 or Ig Isotype control staining versus side light-scatter SSC-A signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Right Panel: PBL collected with the chelating agent, EDTA were similarly treated, stained, and analyzed by multiparameter flow cytometry.