BD PharmingenTM Alexa FluorTM 488 Mouse Anti-Human CD135

Description

Flow cytometric analysis of CD135 expression on human REH cells. Cells from the human REH Acute B cell leukemia, ATCC CRL-8283 cell line were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 557720; dashed line histogram or Alexa Fluor 488 Mouse Anti-Human CD135 antibody Cat. No. 567274/567273; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD135 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Flow cytometric analysis of CD135 expression on human REH cells. Cells from the human REH Acute B cell leukemia, ATCC CRL-8283 cell line were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 557720; dashed line histogram or Alexa Fluor 488 Mouse Anti-Human CD135 antibody Cat. No. 567274/567273; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD135 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software. Show More Flow cytometric analysis of CD135 expression on human REH cells. Cells from the human REH Acute B cell leukemia, ATCC CRL-8283 cell line were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 557720; dashed line histogram or Alexa Fluor 488 Mouse Anti-Human CD135 antibody Cat. No. 567274/567273; solid line histogram . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD135 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative cells. Flow cytometry and data analysis was performed using a BD LSR II Flow Cytometer System and FlowJo software.