BD PharmingenTM Alexa FluorTM 488 Mouse Anti-Human CD138 (Syndecan-1)

Description

Flow cytometric analysis of CD138 Syndecan-1 expression on viable Human U266 myeloma cells. Cells from the Human U266 Myeloma, ATCC TIB-196 cell line were preincubated with Human BD Fc Block Cat. No. 564219 and stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; dashed line histogram or Alexa Fluor 488 Mouse Anti-Human CD138 Syndecan-1 antibody Cat. No. 571513/571563; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD138 Syndecan-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of CD138 Syndecan-1 expression on viable Human U266 myeloma cells. Cells from the Human U266 Myeloma, ATCC TIB-196 cell line were preincubated with Human BD Fc Block Cat. No. 564219 and stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; dashed line histogram or Alexa Fluor 488 Mouse Anti-Human CD138 Syndecan-1 antibody Cat. No. 571513/571563; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD138 Syndecan-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of CD138 Syndecan-1 expression on viable Human U266 myeloma cells. Cells from the Human U266 Myeloma, ATCC TIB-196 cell line were preincubated with Human BD Fc Block Cat. No. 564219 and stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; dashed line histogram or Alexa Fluor 488 Mouse Anti-Human CD138 Syndecan-1 antibody Cat. No. 571513/571563; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing CD138 Syndecan-1 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.