BD PharmingenTM Alexa FluorTM 488 Mouse Anti-Human CD25

Description

Flow cytometric analysis of CD25 expression on PHA-stimulated human peripheral blood lymphocytes. Phytohemagglutinin-stimulated 3 days PBMCs were stained with either Alexa Fluor 488 Mouse Anti-Human CD25 Cat. No. 567474/567475; solid line histogram or Alexa Fluor 488 Mouse IgG1 kappa Isotype Control Cat. No. 565572; dashed line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD25 expression on PHA-stimulated human peripheral blood lymphocytes. Phytohemagglutinin-stimulated 3 days PBMCs were stained with either Alexa Fluor 488 Mouse Anti-Human CD25 Cat. No. 567474/567475; solid line histogram or Alexa Fluor 488 Mouse IgG1 kappa Isotype Control Cat. No. 565572; dashed line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD25 expression on PHA-stimulated human peripheral blood lymphocytes. Phytohemagglutinin-stimulated 3 days PBMCs were stained with either Alexa Fluor 488 Mouse Anti-Human CD25 Cat. No. 567474/567475; solid line histogram or Alexa Fluor 488 Mouse IgG1 kappa Isotype Control Cat. No. 565572; dashed line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software.