BD PharmingenTM Alexa FluorTM 488 Mouse Anti-Human CD273 (PD-L2)

Description

Flow cytometric analysis of CD273 PD-L2 expression on viable Human monocyte-derived mature dendritic cells. Human monocyte-derived mature dendritic cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; dashed line histogram or Alexa Fluor 488 Mouse Anti-Human CD273 PD-L2 antibody Cat. No. 570055/570136; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The fluorescence histogram showing CD273 PD-L2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Flow cytometric analysis of CD273 PD-L2 expression on viable Human monocyte-derived mature dendritic cells. Human monocyte-derived mature dendritic cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; dashed line histogram or Alexa Fluor 488 Mouse Anti-Human CD273 PD-L2 antibody Cat. No. 570055/570136; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The fluorescence histogram showing CD273 PD-L2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Show More Flow cytometric analysis of CD273 PD-L2 expression on viable Human monocyte-derived mature dendritic cells. Human monocyte-derived mature dendritic cells were stained with either Alexa Fluor 488 Mouse IgG1, kappa Isotype Control Cat. No. 565572; dashed line histogram or Alexa Fluor 488 Mouse Anti-Human CD273 PD-L2 antibody Cat. No. 570055/570136; solid line histogram . BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The fluorescence histogram showing CD273 PD-L2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software.